Complement activation in the aqueous humor of pseudophakic bullous keratopathy patients

Ágnes Füst, Dorottya Csuka, Ildikó Süveges, László Imre, Mária Bausz, Attila Nagymihály, Ágnes Csorvási, György Füst, János Németh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background/Aims: According to some studies, inflammation is a potential etiological factor in pseudophakic bullous keratopathy (PBK). Our aim was to obtain information on the activation of the complement system in the aqueous humor in this disorder. Methods: Aqueous humor samples were collected during keratoplasty of 12 PBK patients, as well as during phacoemulsification surgery of 18 control patients. The concentrations of the protein-protein complexes generated during complement activation (C1rC1sC1inh and C3bBbP) through the classical and alternative pathways, respectively, as well as of the C3 cleavage product C3a, were measured with ELISA methods. The correlation among the complement factors and between the duration of the edema, the stage of the disease, and the level of the complement activation products was examined. Results: The concentration of C1rC1sC1inh, C3bBbP complex and C3a was significantly higher in the PBK group (p < 0.001) compared to the control group. In PBK patients, a correlation was found between the levels of the C1rC1sC1inh complex and C3a only. Conclusion: Our new findings indicate that in PBK the complement system is activated-via the classical pathway-in the aqueous humor. The activated complement may play a role in increased endothelial cell loss.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalOphthalmic Research
Volume49
Issue number3
DOIs
Publication statusPublished - Jan 1 2013

Keywords

  • Aqueous humor
  • Complement activation
  • Corneal endothelium
  • Pseudophakic bullous keratopathy

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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