Complement activation in human serum by liposome-encapsulated hemoglobin: The role of natural anti-phospholipid antibodies

Janos Szebeni, Nabila M. Wassef, Alan S. Rudolph, Carl R. Alving

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

In exploring the occurrence and mechanism of liposome-encapsulated hemoglobin (LEH)-induced complement (C) activation, we found that normal human serum contained low titers of IgG and IgM class natural antibodies with reactivity against LEH, and that the amount of vesicle-bound IgM significantly correlated with LEH-induced C consumption. IgM binding to LEH was inhibited by phosphocholine and ATP, but not by choline chloride. These data suggest that naturally occurring antibodies play a key role in LEH-induced C activation, and that a major portion of these antibodies are directed against the phosphate moiety on the phospholipid headgroups of liposome bilayers.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1285
Issue number2
DOIs
Publication statusPublished - Dec 4 1996

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Keywords

  • Antibody
  • Complement
  • Hemoglobin
  • Liposome
  • Natural anti-phospholipid antibody

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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