Complement activation in human serum by liposome-encapsulated hemoglobin

The role of natural anti-phospholipid antibodies

J. Szebeni, Nabila M. Wassef, Alan S. Rudolph, Carl R. Alving

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

In exploring the occurrence and mechanism of liposome-encapsulated hemoglobin (LEH)-induced complement (C) activation, we found that normal human serum contained low titers of IgG and IgM class natural antibodies with reactivity against LEH, and that the amount of vesicle-bound IgM significantly correlated with LEH-induced C consumption. IgM binding to LEH was inhibited by phosphocholine and ATP, but not by choline chloride. These data suggest that naturally occurring antibodies play a key role in LEH-induced C activation, and that a major portion of these antibodies are directed against the phosphate moiety on the phospholipid headgroups of liposome bilayers.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalBBA - Biomembranes
Volume1285
Issue number2
DOIs
Publication statusPublished - Dec 4 1996

Fingerprint

Complement Activation
Liposomes
Anti-Idiotypic Antibodies
Phospholipids
Hemoglobins
Chemical activation
Antibodies
Serum
Hemoglobin C
Immunoglobulin M
Phosphorylcholine
Immunoglobulin Isotypes
Choline
Immunoglobulin G
Adenosine Triphosphate
Phosphates

Keywords

  • Antibody
  • Complement
  • Hemoglobin
  • Liposome
  • Natural anti-phospholipid antibody

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Complement activation in human serum by liposome-encapsulated hemoglobin : The role of natural anti-phospholipid antibodies. / Szebeni, J.; Wassef, Nabila M.; Rudolph, Alan S.; Alving, Carl R.

In: BBA - Biomembranes, Vol. 1285, No. 2, 04.12.1996, p. 127-130.

Research output: Contribution to journalArticle

Szebeni, J. ; Wassef, Nabila M. ; Rudolph, Alan S. ; Alving, Carl R. / Complement activation in human serum by liposome-encapsulated hemoglobin : The role of natural anti-phospholipid antibodies. In: BBA - Biomembranes. 1996 ; Vol. 1285, No. 2. pp. 127-130.
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