Incubation of liposome-encapsulated Hb (LEH) with rat serum at 37C led to accelerated decay of serum hemolytic complement (C) activity (CH50/ml). Empty liposomes (L) caused less decrease of CH50/ml, whereas free Hb had no effect on C activity. The LEH- and L-induced increases in C consumption were unlikely a consequence of endotoxin (LPS) contamination, as spiking of rat serum with LPS caused reduction in C only at levels significantly higher than those detectable in LEH or L. LPS-induced C consumption was not potentiated by free hemoglobin.
|Number of pages||9|
|Journal||Artificial Cells, Blood Substitutes, and Biotechnology|
|Publication status||Published - Jan 1995|
ASJC Scopus subject areas
- Biomedical Engineering