Complement activation by liposome-encapsulated hemoglobin in vitro: The role of endotoxin contamination

Janos Szebeni, Nabila M. Wassef, Alan S. Rudolph, Carl R. Alving

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Incubation of liposome-encapsulated Hb (LEH) with rat serum at 37C led to accelerated decay of serum hemolytic complement (C) activity (CH50/ml). Empty liposomes (L) caused less decrease of CH50/ml, whereas free Hb had no effect on C activity. The LEH- and L-induced increases in C consumption were unlikely a consequence of endotoxin (LPS) contamination, as spiking of rat serum with LPS caused reduction in C only at levels significantly higher than those detectable in LEH or L. LPS-induced C consumption was not potentiated by free hemoglobin.

Original languageEnglish
Pages (from-to)355-363
Number of pages9
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Issue number3
Publication statusPublished - Jan 1995


ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

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