Complement activation by liposome-encapsulated hemoglobin in vitro: The role of endotoxin contamination

J. Szebeni, N. M. Wassef, A. S. Rudolph, C. R. Alving

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Incubation of liposome-encapsulated Hb (LEH) with rat serum at 37°C led to accelerated decay of serum hemolytic complement (C) activity (CH50/ml). Empty liposomes (L) caused less decrease of CH50/ml, whereas free Hb had no effect on C activity. The LEH- and L-induced increases in C consumption were unlikely a consequence of endotoxin (LPS) contamination, as spiking of rat serum with LPS caused reduction in C only at levels significantly higher than those detectable in LEH or L. LPS-induced C consumption was not potentiated by free hemoglobin.

Original languageEnglish
Pages (from-to)355-363
Number of pages9
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume23
Issue number3
Publication statusPublished - 1995

Fingerprint

Liposomes
Complement Activation
Hemoglobin
Endotoxins
Hemoglobins
Contamination
Chemical activation
Rats
Serum
Complement System Proteins
In Vitro Techniques
hemoglobin L

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials
  • Hematology

Cite this

Complement activation by liposome-encapsulated hemoglobin in vitro : The role of endotoxin contamination. / Szebeni, J.; Wassef, N. M.; Rudolph, A. S.; Alving, C. R.

In: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, Vol. 23, No. 3, 1995, p. 355-363.

Research output: Contribution to journalArticle

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