Complement activating antibodies against the human 60 kDa heat shock protein as a new independent family risk factor of coronary heart disease

A. Veres, T. Szamosi, M. Ablonczy, T. Szamosi, M. Singh, I. Karádi, L. Romics, G. Füst, Z. Prohászka

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Several groups have reported high levels of antibodies against 60 kDa heat shock proteins (hsp) associated with coronary heart disease. Methods and results: Complement activating (CA) antihsp60 autoantibodies were measured by the AtheroRisk kit (CardioPath Ltd, Alloa, UK), in parallel with IgG antibodies to human hsp60 and mycobacterial hsp65 by ELISA in 32 healthy children (18 boys, 14 girls, 11.8±4.0 years). At least one of the parents of these children had a history of myocardial infarction before 55 years of age (high family risk (HFR) group). The control group consisted of 63 healthy children (31 boys, 32 girls, 9.0±3.6 years) without known family history of coronary heart disease (CHD), hypertension, and diabetes mellitus. Concentrations of CA antihsp60 antibodies were significantly (P = 0.021) higher in the HFR group than in the control group. Also in the HFR group, significantly (P = 0.004) lower high-density lipoprotein cholesterol (HDL-C)-cholesterol (measured enzymatically) and significantly (P = 0.020) higher low-density lipoprotein cholesterol (LDL-C)-cholesterol levels (calculated by the Friedewald formula) were observed when compared with the controls. The difference in the CA antihsp60 antibody levels between the HFR and control groups remained significant even after adjustments for age, smoking, HDL-cholesterol, LDL-cholesterol levels, and white blood cell count. Children with high (in the highest quartile) CA antihsp60 antibody levels compared with those with normal levels of these antibodies also had adjusted odds ratios (OR) of 9.80 (2.15-44.58,P = 0.003), indicating high family risk. No significant difference in the IgG antihsp antibody levels was observed. Conclusions: These findings indicate that high levels of CA autoantibodies against hsp60 can be considered to be a novel family risk factor of CHD, independent of HDL- and LDL-cholesterol levels.

Original languageEnglish
Pages (from-to)405-410
Number of pages6
JournalEuropean Journal of Clinical Investigation
Volume32
Issue number6
DOIs
Publication statusPublished - 2002

Fingerprint

Chaperonin 60
Heat-Shock Proteins
Coronary Disease
Antibodies
HDL Cholesterol
LDL Cholesterol
Autoantibodies
Control Groups
Immunoglobulin G
Cholesterol
Medical problems
Leukocyte Count
Diabetes Mellitus
Blood
Parents
Smoking
Enzyme-Linked Immunosorbent Assay
Odds Ratio
Myocardial Infarction
Cells

Keywords

  • Children
  • Cholesterol
  • Complement activating antibodies
  • Coronary heart disease
  • Family risk
  • Heat shock protein 60

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Complement activating antibodies against the human 60 kDa heat shock protein as a new independent family risk factor of coronary heart disease. / Veres, A.; Szamosi, T.; Ablonczy, M.; Szamosi, T.; Singh, M.; Karádi, I.; Romics, L.; Füst, G.; Prohászka, Z.

In: European Journal of Clinical Investigation, Vol. 32, No. 6, 2002, p. 405-410.

Research output: Contribution to journalArticle

@article{af6726cb007542479f23a356bc11d2d3,
title = "Complement activating antibodies against the human 60 kDa heat shock protein as a new independent family risk factor of coronary heart disease",
abstract = "Background: Several groups have reported high levels of antibodies against 60 kDa heat shock proteins (hsp) associated with coronary heart disease. Methods and results: Complement activating (CA) antihsp60 autoantibodies were measured by the AtheroRisk kit (CardioPath Ltd, Alloa, UK), in parallel with IgG antibodies to human hsp60 and mycobacterial hsp65 by ELISA in 32 healthy children (18 boys, 14 girls, 11.8±4.0 years). At least one of the parents of these children had a history of myocardial infarction before 55 years of age (high family risk (HFR) group). The control group consisted of 63 healthy children (31 boys, 32 girls, 9.0±3.6 years) without known family history of coronary heart disease (CHD), hypertension, and diabetes mellitus. Concentrations of CA antihsp60 antibodies were significantly (P = 0.021) higher in the HFR group than in the control group. Also in the HFR group, significantly (P = 0.004) lower high-density lipoprotein cholesterol (HDL-C)-cholesterol (measured enzymatically) and significantly (P = 0.020) higher low-density lipoprotein cholesterol (LDL-C)-cholesterol levels (calculated by the Friedewald formula) were observed when compared with the controls. The difference in the CA antihsp60 antibody levels between the HFR and control groups remained significant even after adjustments for age, smoking, HDL-cholesterol, LDL-cholesterol levels, and white blood cell count. Children with high (in the highest quartile) CA antihsp60 antibody levels compared with those with normal levels of these antibodies also had adjusted odds ratios (OR) of 9.80 (2.15-44.58,P = 0.003), indicating high family risk. No significant difference in the IgG antihsp antibody levels was observed. Conclusions: These findings indicate that high levels of CA autoantibodies against hsp60 can be considered to be a novel family risk factor of CHD, independent of HDL- and LDL-cholesterol levels.",
keywords = "Children, Cholesterol, Complement activating antibodies, Coronary heart disease, Family risk, Heat shock protein 60",
author = "A. Veres and T. Szamosi and M. Ablonczy and T. Szamosi and M. Singh and I. Kar{\'a}di and L. Romics and G. F{\"u}st and Z. Proh{\'a}szka",
year = "2002",
doi = "10.1046/j.1365-2362.2002.01007.x",
language = "English",
volume = "32",
pages = "405--410",
journal = "European Journal of Clinical Investigation",
issn = "0014-2972",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Complement activating antibodies against the human 60 kDa heat shock protein as a new independent family risk factor of coronary heart disease

AU - Veres, A.

AU - Szamosi, T.

AU - Ablonczy, M.

AU - Szamosi, T.

AU - Singh, M.

AU - Karádi, I.

AU - Romics, L.

AU - Füst, G.

AU - Prohászka, Z.

PY - 2002

Y1 - 2002

N2 - Background: Several groups have reported high levels of antibodies against 60 kDa heat shock proteins (hsp) associated with coronary heart disease. Methods and results: Complement activating (CA) antihsp60 autoantibodies were measured by the AtheroRisk kit (CardioPath Ltd, Alloa, UK), in parallel with IgG antibodies to human hsp60 and mycobacterial hsp65 by ELISA in 32 healthy children (18 boys, 14 girls, 11.8±4.0 years). At least one of the parents of these children had a history of myocardial infarction before 55 years of age (high family risk (HFR) group). The control group consisted of 63 healthy children (31 boys, 32 girls, 9.0±3.6 years) without known family history of coronary heart disease (CHD), hypertension, and diabetes mellitus. Concentrations of CA antihsp60 antibodies were significantly (P = 0.021) higher in the HFR group than in the control group. Also in the HFR group, significantly (P = 0.004) lower high-density lipoprotein cholesterol (HDL-C)-cholesterol (measured enzymatically) and significantly (P = 0.020) higher low-density lipoprotein cholesterol (LDL-C)-cholesterol levels (calculated by the Friedewald formula) were observed when compared with the controls. The difference in the CA antihsp60 antibody levels between the HFR and control groups remained significant even after adjustments for age, smoking, HDL-cholesterol, LDL-cholesterol levels, and white blood cell count. Children with high (in the highest quartile) CA antihsp60 antibody levels compared with those with normal levels of these antibodies also had adjusted odds ratios (OR) of 9.80 (2.15-44.58,P = 0.003), indicating high family risk. No significant difference in the IgG antihsp antibody levels was observed. Conclusions: These findings indicate that high levels of CA autoantibodies against hsp60 can be considered to be a novel family risk factor of CHD, independent of HDL- and LDL-cholesterol levels.

AB - Background: Several groups have reported high levels of antibodies against 60 kDa heat shock proteins (hsp) associated with coronary heart disease. Methods and results: Complement activating (CA) antihsp60 autoantibodies were measured by the AtheroRisk kit (CardioPath Ltd, Alloa, UK), in parallel with IgG antibodies to human hsp60 and mycobacterial hsp65 by ELISA in 32 healthy children (18 boys, 14 girls, 11.8±4.0 years). At least one of the parents of these children had a history of myocardial infarction before 55 years of age (high family risk (HFR) group). The control group consisted of 63 healthy children (31 boys, 32 girls, 9.0±3.6 years) without known family history of coronary heart disease (CHD), hypertension, and diabetes mellitus. Concentrations of CA antihsp60 antibodies were significantly (P = 0.021) higher in the HFR group than in the control group. Also in the HFR group, significantly (P = 0.004) lower high-density lipoprotein cholesterol (HDL-C)-cholesterol (measured enzymatically) and significantly (P = 0.020) higher low-density lipoprotein cholesterol (LDL-C)-cholesterol levels (calculated by the Friedewald formula) were observed when compared with the controls. The difference in the CA antihsp60 antibody levels between the HFR and control groups remained significant even after adjustments for age, smoking, HDL-cholesterol, LDL-cholesterol levels, and white blood cell count. Children with high (in the highest quartile) CA antihsp60 antibody levels compared with those with normal levels of these antibodies also had adjusted odds ratios (OR) of 9.80 (2.15-44.58,P = 0.003), indicating high family risk. No significant difference in the IgG antihsp antibody levels was observed. Conclusions: These findings indicate that high levels of CA autoantibodies against hsp60 can be considered to be a novel family risk factor of CHD, independent of HDL- and LDL-cholesterol levels.

KW - Children

KW - Cholesterol

KW - Complement activating antibodies

KW - Coronary heart disease

KW - Family risk

KW - Heat shock protein 60

UR - http://www.scopus.com/inward/record.url?scp=0036997958&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036997958&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2362.2002.01007.x

DO - 10.1046/j.1365-2362.2002.01007.x

M3 - Article

VL - 32

SP - 405

EP - 410

JO - European Journal of Clinical Investigation

JF - European Journal of Clinical Investigation

SN - 0014-2972

IS - 6

ER -