Competition between transposable elements and mutator genes in bacteria

Tamás Fehér, Balázs Bogos, Orsolya Méhi, Gergely Fekete, Bálint Csörg, Károly Kovács, G. Pósfai, B. Papp, Laurence D. Hurst, C. Pál

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Although both genotypes with elevated mutation rate (mutators) and mobilization of insertion sequence (IS) elements have substantial impact on genome diversification, their potential interactions are unknown. Moreover, the evolutionary forces driving gradual accumulation of these elements are unclear: Do these elements spread in an initially transposon-free bacterial genome as they enable rapid adaptive evolution? To address these issues, we inserted an active IS1 element into a reduced Escherichia coli genome devoid of all other mobile DNA. Evolutionary laboratory experiments revealed that IS elements increase mutational supply and occasionally generate variants with especially large phenotypic effects. However, their impact on adaptive evolution is small compared with mismatch repair mutator alleles, and hence, the latter impede the spread of IS-carrying strains. Given their ubiquity in natural populations, such mutator alleles could limit early phase of IS element evolution in a new bacterial host. More generally, our work demonstrates the existence of an evolutionary conflict between mutation-promoting mechanisms.

Original languageEnglish
Pages (from-to)3153-3159
Number of pages7
JournalMolecular Biology and Evolution
Volume29
Issue number10
DOIs
Publication statusPublished - Oct 2012

Fingerprint

DNA Transposable Elements
Mutation Rate
transposons
mutation
Bacteria
bacterium
genome
gene
bacteria
Alleles
Genome
Genes
Bacterial Genomes
allele
DNA Mismatch Repair
genes
Insertional Mutagenesis
alleles
Genotype
Escherichia coli

Keywords

  • evolution
  • IS elements
  • mutation rate

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Competition between transposable elements and mutator genes in bacteria. / Fehér, Tamás; Bogos, Balázs; Méhi, Orsolya; Fekete, Gergely; Csörg, Bálint; Kovács, Károly; Pósfai, G.; Papp, B.; Hurst, Laurence D.; Pál, C.

In: Molecular Biology and Evolution, Vol. 29, No. 10, 10.2012, p. 3153-3159.

Research output: Contribution to journalArticle

Fehér, T, Bogos, B, Méhi, O, Fekete, G, Csörg, B, Kovács, K, Pósfai, G, Papp, B, Hurst, LD & Pál, C 2012, 'Competition between transposable elements and mutator genes in bacteria', Molecular Biology and Evolution, vol. 29, no. 10, pp. 3153-3159. https://doi.org/10.1093/molbev/mss122
Fehér T, Bogos B, Méhi O, Fekete G, Csörg B, Kovács K et al. Competition between transposable elements and mutator genes in bacteria. Molecular Biology and Evolution. 2012 Oct;29(10):3153-3159. https://doi.org/10.1093/molbev/mss122
Fehér, Tamás ; Bogos, Balázs ; Méhi, Orsolya ; Fekete, Gergely ; Csörg, Bálint ; Kovács, Károly ; Pósfai, G. ; Papp, B. ; Hurst, Laurence D. ; Pál, C. / Competition between transposable elements and mutator genes in bacteria. In: Molecular Biology and Evolution. 2012 ; Vol. 29, No. 10. pp. 3153-3159.
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