Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm

G. Bernáth, Z. Bokor, E. Kása, L. Várkonyi, T. Hegyi, T. Kollár, B. Urbányi, D. Zarski, J. Radóczi Ifj., T. Horváth

Research output: Contribution to journalArticle

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Abstract

Two different cryopreservation methods were compared and an optimal dilution ratio for the use of controlled-rate freezer (CRF) was established for Eurasian perch (Perca fluviatilis) sperm. Progressive motility (72. ±. 15%) and curvilinear velocity (VCL, 146. ±. 11. μm/s) of sperm cryopreserved with CRF did not reduce significantly compared to fresh sperm [progressive motility (90. ±. 4%), VCL (173. ±. 24. μm/s)]. On the other hand, progressive motility (62. ±. 15%) and VCL (120. ±. 21. μm/s) of sperm cryopreserved with the conventional floating frame technique were significantly lower when compared to the fresh control. Sperm in both cryopreserved groups showed significantly higher straightness [STR, CRF (84. ±. 4%), frame (84. ±. 2%)] than in the fresh control group (68. ±. 4%). Perch sperm cryopreserved with CRF at a dilution ratio of 1:20 showed significantly higher progressive motility (49. ±. 6%) than at a ratio of 1:5 (39. ±. 6%) and showed significantly higher VCL (129. ±. 11. μm/s) than at dilution ratios of 1:10 (112. ±. 17. μm/s) and 1:5 (115. ±. 9. μm/s).

Original languageEnglish
Pages (from-to)76-78
Number of pages3
JournalCryobiology
Volume70
Issue number1
DOIs
Publication statusPublished - Feb 1 2015

Fingerprint

Perches
Perca fluviatilis
Cryopreservation
cryopreservation
Dilution
Spermatozoa
freezers
spermatozoa
methodology
Sperm Motility
perches (animal housing)
Control Groups

Keywords

  • Controlled-rate freezer
  • Cryopreservation
  • Eurasian perch
  • Sperm
  • Standardization

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Bernáth, G., Bokor, Z., Kása, E., Várkonyi, L., Hegyi, T., Kollár, T., ... Horváth, T. (2015). Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm. Cryobiology, 70(1), 76-78. https://doi.org/10.1016/j.cryobiol.2014.12.003

Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm. / Bernáth, G.; Bokor, Z.; Kása, E.; Várkonyi, L.; Hegyi, T.; Kollár, T.; Urbányi, B.; Zarski, D.; Radóczi Ifj., J.; Horváth, T.

In: Cryobiology, Vol. 70, No. 1, 01.02.2015, p. 76-78.

Research output: Contribution to journalArticle

Bernáth, G, Bokor, Z, Kása, E, Várkonyi, L, Hegyi, T, Kollár, T, Urbányi, B, Zarski, D, Radóczi Ifj., J & Horváth, T 2015, 'Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm', Cryobiology, vol. 70, no. 1, pp. 76-78. https://doi.org/10.1016/j.cryobiol.2014.12.003
Bernáth, G. ; Bokor, Z. ; Kása, E. ; Várkonyi, L. ; Hegyi, T. ; Kollár, T. ; Urbányi, B. ; Zarski, D. ; Radóczi Ifj., J. ; Horváth, T. / Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm. In: Cryobiology. 2015 ; Vol. 70, No. 1. pp. 76-78.
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AU - Hegyi, T.

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