Comparison of thirteen methods for aDNA (1731-1841) extractions

Emese Juhász, K. Nagy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The aim of this Chapter is to compare thirteen different DNA extraction methods for the isolation of PCR amplifiable aDNAs from mummified soft tissues recovered from 1731-1841 (Vác, Hungary). Among the techniques and methods, the silicabased guanidium thiocyanate treatment and the phenol/chloroform extraction method combined with PTB (N-phenacyl-thiazolium bromide) were found to be powerful for aDNA isolation. These results might be indicative for Plant Archaeogenetists.

Original languageEnglish
Title of host publicationPlant Archaeogenetics
PublisherNova Science Publishers, Inc.
Pages129-134
Number of pages6
ISBN (Print)9781611226447
Publication statusPublished - 2011

Fingerprint

thiocyanates
Hungary
isolation techniques
Chloroform
Phenol
bromides
chloroform
phenol
methodology
Polymerase Chain Reaction
DNA
tissues
thiocyanate
phenacyl bromide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Juhász, E., & Nagy, K. (2011). Comparison of thirteen methods for aDNA (1731-1841) extractions. In Plant Archaeogenetics (pp. 129-134). Nova Science Publishers, Inc..

Comparison of thirteen methods for aDNA (1731-1841) extractions. / Juhász, Emese; Nagy, K.

Plant Archaeogenetics. Nova Science Publishers, Inc., 2011. p. 129-134.

Research output: Chapter in Book/Report/Conference proceedingChapter

Juhász, E & Nagy, K 2011, Comparison of thirteen methods for aDNA (1731-1841) extractions. in Plant Archaeogenetics. Nova Science Publishers, Inc., pp. 129-134.
Juhász E, Nagy K. Comparison of thirteen methods for aDNA (1731-1841) extractions. In Plant Archaeogenetics. Nova Science Publishers, Inc. 2011. p. 129-134
Juhász, Emese ; Nagy, K. / Comparison of thirteen methods for aDNA (1731-1841) extractions. Plant Archaeogenetics. Nova Science Publishers, Inc., 2011. pp. 129-134
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