Comparison of thin-layer chromatography and overpressured layer chromatographic techniques for the separation of ascorbigen and 1'-methylascorbigen

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Abstract

Simple and efficient methods are described for the separation of biologically active ascorbigen and 1'-methylascorbigen by normal-phase TLC, conventional as well as new personal overpressured layer chromatographic (OPLC) systems using the same eluent. Results obtained with these techniques were compared on the basis of the separation parameters with special emphasis on the presentation of the advantages of the automated personal OPLC system. For the characterization of the personal OPLC system dependence of average plate height and resolution on the external pressure, front velocity and development distance was examined on TLC and HPTLC sorbent layers using ascorbigen and 1'-methylascorbigen as model compounds.

Original languageEnglish
Pages (from-to)103-109
Number of pages7
JournalJournal of Chromatography A
Volume764
Issue number1
DOIs
Publication statusPublished - Mar 7 1997

Keywords

  • Ascorbigen
  • Methylascorbigen

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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