Comparison of the tumorigenic potential of liver and kidney tumors induced by N-nitrosodimethylamine

Gyorgy Trencsenyi, Tamas Juhasz, Fruzsina Bako, T. Márián, I. Pócsi, Pal Kertai, J. Hunyadi, G. Bánfalvi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of the study was to determine the tumorigenic potential of two cell lines established from N-nitrosodimethylamine induced rat hepatocarcinoma (HeDe) and mesenchymal renal tumors (NeDe). The basis of the distinction is that human cancers are known to overexpress facilitative GLUT transporters and TGFβ1 protein. These proteins are linked to the increased metabolic energy consumption indicating uncontrolled growth and proliferation. We have assayed not only the expression of GLUT-1, GLUT-3 and TGF-β1 proteins, but also the uptake of 2-fluoro-[18F]-2-deoxi-D-glucose (18FDG), a tracer for cancer diagnosis. Western blot analysis and whole body autoradiography were used to measure the 18FDG uptake of tumor cells. Elevated 18FDG uptake was measured in both tumor cell lines. Whole body autoradiography provided evidence that the uptake of 18FDG was lower in the necrotic inner part than in the more vascularized outer parts of primary hepatocarcinoma and mesenchymal renal tumors. GLUT-1 overexpression in hepatocarcinoma tumor, and high levels of GLUT-3 were found in the NeDe cell line and in the mesenchymal renal tumor. TGF-β-1 was overexpressed in hepatocarcinoma and mesenchymal renal tumors. In vitro and in vivo parameters support the view that the tumorigenic potential of cancer cells cannot be determined by the expression of a single parameter such as the expression of either GLUT-1, GLUT-3 or 18FDG uptake. Besides the tumorigenic potential of the hepatocarcinoma, the high metabolic activity of the renal tumor indicated by its 18FDG uptake, GLUT-3 and TGF-β1 expression, the mesenchymal renal tumor induced by N-nitrosodimethylamine is not a benign, but an an aggressive renal carcinoma.

Original languageEnglish
Pages (from-to)309-320
Number of pages12
JournalHistology and Histopathology
Volume25
Issue number3
Publication statusPublished - Mar 2010

Fingerprint

Dimethylnitrosamine
Kidney
Liver
Neoplasms
Glucose
Autoradiography
Cell Line
Proteins
Tumor Cell Line
Western Blotting

Keywords

  • 18FDG ptake
  • Chemical induction
  • Gene expression
  • GLUT transporters
  • Primary tumor
  • Tumor cell lines
  • Whole body autoradiography

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Comparison of the tumorigenic potential of liver and kidney tumors induced by N-nitrosodimethylamine. / Trencsenyi, Gyorgy; Juhasz, Tamas; Bako, Fruzsina; Márián, T.; Pócsi, I.; Kertai, Pal; Hunyadi, J.; Bánfalvi, G.

In: Histology and Histopathology, Vol. 25, No. 3, 03.2010, p. 309-320.

Research output: Contribution to journalArticle

@article{c064f9cba1e24d218fca4f81d8a25403,
title = "Comparison of the tumorigenic potential of liver and kidney tumors induced by N-nitrosodimethylamine",
abstract = "The aim of the study was to determine the tumorigenic potential of two cell lines established from N-nitrosodimethylamine induced rat hepatocarcinoma (HeDe) and mesenchymal renal tumors (NeDe). The basis of the distinction is that human cancers are known to overexpress facilitative GLUT transporters and TGFβ1 protein. These proteins are linked to the increased metabolic energy consumption indicating uncontrolled growth and proliferation. We have assayed not only the expression of GLUT-1, GLUT-3 and TGF-β1 proteins, but also the uptake of 2-fluoro-[18F]-2-deoxi-D-glucose (18FDG), a tracer for cancer diagnosis. Western blot analysis and whole body autoradiography were used to measure the 18FDG uptake of tumor cells. Elevated 18FDG uptake was measured in both tumor cell lines. Whole body autoradiography provided evidence that the uptake of 18FDG was lower in the necrotic inner part than in the more vascularized outer parts of primary hepatocarcinoma and mesenchymal renal tumors. GLUT-1 overexpression in hepatocarcinoma tumor, and high levels of GLUT-3 were found in the NeDe cell line and in the mesenchymal renal tumor. TGF-β-1 was overexpressed in hepatocarcinoma and mesenchymal renal tumors. In vitro and in vivo parameters support the view that the tumorigenic potential of cancer cells cannot be determined by the expression of a single parameter such as the expression of either GLUT-1, GLUT-3 or 18FDG uptake. Besides the tumorigenic potential of the hepatocarcinoma, the high metabolic activity of the renal tumor indicated by its 18FDG uptake, GLUT-3 and TGF-β1 expression, the mesenchymal renal tumor induced by N-nitrosodimethylamine is not a benign, but an an aggressive renal carcinoma.",
keywords = "18FDG ptake, Chemical induction, Gene expression, GLUT transporters, Primary tumor, Tumor cell lines, Whole body autoradiography",
author = "Gyorgy Trencsenyi and Tamas Juhasz and Fruzsina Bako and T. M{\'a}ri{\'a}n and I. P{\'o}csi and Pal Kertai and J. Hunyadi and G. B{\'a}nfalvi",
year = "2010",
month = "3",
language = "English",
volume = "25",
pages = "309--320",
journal = "Histology and Histopathology",
issn = "0213-3911",
publisher = "Histology and Histopathology",
number = "3",

}

TY - JOUR

T1 - Comparison of the tumorigenic potential of liver and kidney tumors induced by N-nitrosodimethylamine

AU - Trencsenyi, Gyorgy

AU - Juhasz, Tamas

AU - Bako, Fruzsina

AU - Márián, T.

AU - Pócsi, I.

AU - Kertai, Pal

AU - Hunyadi, J.

AU - Bánfalvi, G.

PY - 2010/3

Y1 - 2010/3

N2 - The aim of the study was to determine the tumorigenic potential of two cell lines established from N-nitrosodimethylamine induced rat hepatocarcinoma (HeDe) and mesenchymal renal tumors (NeDe). The basis of the distinction is that human cancers are known to overexpress facilitative GLUT transporters and TGFβ1 protein. These proteins are linked to the increased metabolic energy consumption indicating uncontrolled growth and proliferation. We have assayed not only the expression of GLUT-1, GLUT-3 and TGF-β1 proteins, but also the uptake of 2-fluoro-[18F]-2-deoxi-D-glucose (18FDG), a tracer for cancer diagnosis. Western blot analysis and whole body autoradiography were used to measure the 18FDG uptake of tumor cells. Elevated 18FDG uptake was measured in both tumor cell lines. Whole body autoradiography provided evidence that the uptake of 18FDG was lower in the necrotic inner part than in the more vascularized outer parts of primary hepatocarcinoma and mesenchymal renal tumors. GLUT-1 overexpression in hepatocarcinoma tumor, and high levels of GLUT-3 were found in the NeDe cell line and in the mesenchymal renal tumor. TGF-β-1 was overexpressed in hepatocarcinoma and mesenchymal renal tumors. In vitro and in vivo parameters support the view that the tumorigenic potential of cancer cells cannot be determined by the expression of a single parameter such as the expression of either GLUT-1, GLUT-3 or 18FDG uptake. Besides the tumorigenic potential of the hepatocarcinoma, the high metabolic activity of the renal tumor indicated by its 18FDG uptake, GLUT-3 and TGF-β1 expression, the mesenchymal renal tumor induced by N-nitrosodimethylamine is not a benign, but an an aggressive renal carcinoma.

AB - The aim of the study was to determine the tumorigenic potential of two cell lines established from N-nitrosodimethylamine induced rat hepatocarcinoma (HeDe) and mesenchymal renal tumors (NeDe). The basis of the distinction is that human cancers are known to overexpress facilitative GLUT transporters and TGFβ1 protein. These proteins are linked to the increased metabolic energy consumption indicating uncontrolled growth and proliferation. We have assayed not only the expression of GLUT-1, GLUT-3 and TGF-β1 proteins, but also the uptake of 2-fluoro-[18F]-2-deoxi-D-glucose (18FDG), a tracer for cancer diagnosis. Western blot analysis and whole body autoradiography were used to measure the 18FDG uptake of tumor cells. Elevated 18FDG uptake was measured in both tumor cell lines. Whole body autoradiography provided evidence that the uptake of 18FDG was lower in the necrotic inner part than in the more vascularized outer parts of primary hepatocarcinoma and mesenchymal renal tumors. GLUT-1 overexpression in hepatocarcinoma tumor, and high levels of GLUT-3 were found in the NeDe cell line and in the mesenchymal renal tumor. TGF-β-1 was overexpressed in hepatocarcinoma and mesenchymal renal tumors. In vitro and in vivo parameters support the view that the tumorigenic potential of cancer cells cannot be determined by the expression of a single parameter such as the expression of either GLUT-1, GLUT-3 or 18FDG uptake. Besides the tumorigenic potential of the hepatocarcinoma, the high metabolic activity of the renal tumor indicated by its 18FDG uptake, GLUT-3 and TGF-β1 expression, the mesenchymal renal tumor induced by N-nitrosodimethylamine is not a benign, but an an aggressive renal carcinoma.

KW - 18FDG ptake

KW - Chemical induction

KW - Gene expression

KW - GLUT transporters

KW - Primary tumor

KW - Tumor cell lines

KW - Whole body autoradiography

UR - http://www.scopus.com/inward/record.url?scp=77749331749&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77749331749&partnerID=8YFLogxK

M3 - Article

C2 - 20054803

AN - SCOPUS:77749331749

VL - 25

SP - 309

EP - 320

JO - Histology and Histopathology

JF - Histology and Histopathology

SN - 0213-3911

IS - 3

ER -