Comparison of the retention behaviour of phenol derivatives on porous graphitized and octadecylsilica columns

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The retention of 22 ring-substituted phenol derivatives on porous graphitized carbon (PGC) (eluents: acetonitrile - water and methanol - water mixtures) and on octadecylsilica (ODS) (eluents: methanol - 0.025 M KH2PO4 mixtures) was determined, and the relationship between retention and physicochemical parameters were evaluated by principal component analysis followed by two-dimensional nonlinear mapping and by cluster analysis as well as by canonical correlation analysis. Calculations proved that marked differences can be detected between the retention characteristics of PGC and ODS columns, and the electronic parameters of phenol derivatives have the highest impact on their retention. The comparison of various multivariate mathematical-statistical methods indicated that principal component analysis followed by two dimensional non-linear mapping is the most appropriate method for the evaluation of large data matrices in RP-HPLC.

Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalChromatographia
Volume36
Issue number1
DOIs
Publication statusPublished - Dec 1 1993

    Fingerprint

Keywords

  • Column liquid chromatography
  • Mathematical data analysis
  • ODS and graphitized carbon columns
  • Phenol derivatives
  • Structure-retention relationships

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this