Comparison of the redox activities in plasma membranes from roots and shoots of etiolated bean seedlings

H. Asard, A. Bérczi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Plasma membrane (PM) vesicles were purified in parallel from the roots and shoots of 6-day-old etiolated bean (Phaseolus vulgaris L.) seedlings, grown in water culture at 25 °C, by aqueous polymer two-phase partitioning. The purity of PM fractions was determined by measuring the activity of known marker enzymes (vanadate-sensitive Mg-ATPase, 1,3-β-glycan synthase, latent ID-Pase, cytochrome c oxidase, and antimycin-A-insensitive cytochrome c reductase). NADH-(acceptor) oxidoreductase activities were determined with the following electron acceptors: ferricyanide, cytochrome c, duroquinone, monodehydroascorbate, Fe3+-EDTA, and oxygen. Cytochrome b content was also determined. In general, results show that redox activities are higher in the root PM than in the shoot PM which follows the glycan synthase II (PM marker) pattern. The relative activities of the distinct redox enzymes measured (activity profile) are remarkably similar in the root and shoot PM preparations. The cytochrome b content and level of ascorbate reduction were also similar in both plant organs. However, the ratio of NADH-(acceptor) oxidoreductase activity to cytochrome content was significantly higher in roots when compared to the shoots.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalProtoplasma
Volume205
Issue number1-4
DOIs
Publication statusPublished - Jan 1 1998

Keywords

  • Bean seedling
  • Marker enzymes
  • NADH-(acceptor) oxidoreductase
  • Plasma membrane
  • Root
  • Shoot

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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