Comparison of the kinetic performance of different columns for fast liquid chromatography, emphasizing the contributions of column end structure

Nándor Lambert, Shota Miyazaki, Masayoshi Ohira, Nobuo Tanaka, A. Felinger

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The kinetic performance of five chromatographic columns designed for fast liquid chromatography with different column packing materials – including fully porous (2.0 and 1.9 μm particles), core–shell (2.6 μm particles) or monolithic packings – with identical column dimensions (2.1 × 50 mm) was tested. Since the tested monolithic column showed systematically better efficiency for early eluting compounds than the packed columns, an additional band broadening effect was suspected for the packed columns. The effects of the presence of the frits and the bed heterogeneity of the columns near the frits were characterized by a column-reversal method. It has been shown that significant differences – even 20–25% difference in efficiency – can exist between the two ends of the packed columns, while the monolithic column shows rather similar performance at either column end.

Original languageEnglish
Pages (from-to)99-108
Number of pages10
JournalJournal of Chromatography A
Volume1473
DOIs
Publication statusPublished - Nov 18 2016

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Liquid chromatography
Liquid Chromatography
Kinetics

Keywords

  • Column efficiency
  • Flow reversal
  • Kinetic performance
  • Monolith column
  • UHPLC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Comparison of the kinetic performance of different columns for fast liquid chromatography, emphasizing the contributions of column end structure. / Lambert, Nándor; Miyazaki, Shota; Ohira, Masayoshi; Tanaka, Nobuo; Felinger, A.

In: Journal of Chromatography A, Vol. 1473, 18.11.2016, p. 99-108.

Research output: Contribution to journalArticle

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