Comparison of the HIV-1 and HIV-2 proteinases using oligopeptide substrates representing cleavage sites in Gag and Gag-Pol polyproteins

J. Tőzsér, Ivo Bláha, Terry D. Copeland, Ewald M. Wondrak, Stephen Oroszlan

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

The substrate specificity of the human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) proteinases was compared using oligopeptides corresponding to cleavage sites in the Gag and Gag-Pol polyproteins of both viruses. All peptides mimicking cleavage sites at the junction of major functional protein domains were correctly cleaved by both enzymes. However, some other peptides thought to represent secondary cleavage sites remained intact. The kinetic parameters (Km and km) obtained for the different substrates showed several hundred-fold variation but were similar for the same substrate.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
JournalFEBS Letters
Volume281
Issue number1-2
DOIs
Publication statusPublished - Apr 9 1991

Fingerprint

pol Gene Products
gag Gene Products
Oligopeptides
HIV-2
Viruses
HIV-1
Peptide Hydrolases
Peptides
Substrates
Substrate Specificity
Kinetic parameters
Enzymes
Proteins

Keywords

  • Enzyme kinetics
  • HIV-1 proteinase
  • HIV-2 proteinase
  • Oligopeptide substrate
  • Substrate specificity

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Comparison of the HIV-1 and HIV-2 proteinases using oligopeptide substrates representing cleavage sites in Gag and Gag-Pol polyproteins. / Tőzsér, J.; Bláha, Ivo; Copeland, Terry D.; Wondrak, Ewald M.; Oroszlan, Stephen.

In: FEBS Letters, Vol. 281, No. 1-2, 09.04.1991, p. 77-80.

Research output: Contribution to journalArticle

Tőzsér, J. ; Bláha, Ivo ; Copeland, Terry D. ; Wondrak, Ewald M. ; Oroszlan, Stephen. / Comparison of the HIV-1 and HIV-2 proteinases using oligopeptide substrates representing cleavage sites in Gag and Gag-Pol polyproteins. In: FEBS Letters. 1991 ; Vol. 281, No. 1-2. pp. 77-80.
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