Comparison of the genome of ovine adenovirus types 1 through 5 by restriction enzyme analysis and DNA hybridisation

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5 Citations (Scopus)

Abstract

The DNA of the prototype strains of ovine adenovirus (OAdV) 1 through 5 was analysed by restriction enzyme (RE) digestion. The RE patterns generated by HindIII and PstI enzymes were characteristic of the examined strains. OAdV-2 and 3 resembled each other the most, and their EcoRI and HindIII patterns seemed to be identical. Considering the number of comigrating fragments, serotypes OAdV-2, 3, 4 and 5 looked more closely related to each other than to OAdV-1. This finding was strengthened by Southern blot hybridisations probed with random HindIII clones of OAdV-3. The estimated genome size of the examined OAdV types ranged between 31.9 and 32.8 kilobase pairs. The results supported the new genus classification of OAdVs.

Original languageEnglish
Pages (from-to)477-484
Number of pages8
JournalActa Veterinaria Hungarica
Volume48
Issue number4
Publication statusPublished - 2000

Fingerprint

Ovine adenovirus A
Restriction Mapping
restriction mapping
Adenoviridae
nucleic acid hybridization
Ovine adenovirus B
Sheep
Genome
sheep
genome
DNA
enzymes
Enzymes
prototypes
Southern blotting
Genome Size
new genus
serotypes
hybridization
digestion

Keywords

  • Ovine adenoviruses
  • Restriction enzyme analysis
  • Southern hybridisation
  • Taxonomy

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "Comparison of the genome of ovine adenovirus types 1 through 5 by restriction enzyme analysis and DNA hybridisation",
abstract = "The DNA of the prototype strains of ovine adenovirus (OAdV) 1 through 5 was analysed by restriction enzyme (RE) digestion. The RE patterns generated by HindIII and PstI enzymes were characteristic of the examined strains. OAdV-2 and 3 resembled each other the most, and their EcoRI and HindIII patterns seemed to be identical. Considering the number of comigrating fragments, serotypes OAdV-2, 3, 4 and 5 looked more closely related to each other than to OAdV-1. This finding was strengthened by Southern blot hybridisations probed with random HindIII clones of OAdV-3. The estimated genome size of the examined OAdV types ranged between 31.9 and 32.8 kilobase pairs. The results supported the new genus classification of OAdVs.",
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AU - Benkó, M.

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AB - The DNA of the prototype strains of ovine adenovirus (OAdV) 1 through 5 was analysed by restriction enzyme (RE) digestion. The RE patterns generated by HindIII and PstI enzymes were characteristic of the examined strains. OAdV-2 and 3 resembled each other the most, and their EcoRI and HindIII patterns seemed to be identical. Considering the number of comigrating fragments, serotypes OAdV-2, 3, 4 and 5 looked more closely related to each other than to OAdV-1. This finding was strengthened by Southern blot hybridisations probed with random HindIII clones of OAdV-3. The estimated genome size of the examined OAdV types ranged between 31.9 and 32.8 kilobase pairs. The results supported the new genus classification of OAdVs.

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KW - Taxonomy

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