Comparison of the effects of selected chalcones, dihydrochalcones and some cyclic flavonoids on mitochondrial outer membrane determined by fluorescence spectroscopy

Vladimíra Tomečková, Juraj Guzy, Jaroslav Kušnír, Krisztina Fodor, Mária Mareková, Zenóbia Chavková, Pál Perjési

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The effect on mitochondrial outer membrane of 4-hydroxychalcone (1), the cyclic chalcone analogues E-2-(4′-hydroxybenzylidene)-1-indanone (2a) and E-2-(4′-hydroxybenzylidene)-1-tetralone (2b), the dihydrochalcones phloretin (3a) and phloridzin (3b), the flavanones naringenin (4a) and naringin (4b), and the flavonol quercetin (5) was investigated by fluorescence spectroscopy. Excitation and emission fluorescence spectra of each flavonoid and synthetic analogue were recorded in respiration medium containing 1 mM succinate. Initial interaction of the compounds with the outer mitochondrial membrane was investigated by recording their fluorescence polarization in the presence of rat liver mitochondria. Most of the compounds displayed an elevated fluorescence polarization on mixing with mitochondria at the zero time point. During the investigated 20 min period the initial fluorescence polarization values remained constant (1, 2a), or a gradual depression of the measured polarization values could be observed (2b, 3a, 4b, 5). In the case of naringenin (4a), however, similar to the previously investigated seven-membered cyclic chalcone analogue E-2-(4 ́-methoxybenzylidene)-1-benzosuberone, a slight, continuous increase of fluorescence polarization could be detected during the 20 min experiment. Phloridzin (3b) showed an increased fluorescence polarization in first 10 min, which was slightly depressed by the 20 min time point.

Original languageEnglish
Pages (from-to)143-150
Number of pages8
JournalJournal of biochemical and biophysical methods
Volume69
Issue number1-2
DOIs
Publication statusPublished - Nov 30 2006

Keywords

  • Chalcones
  • Dihydrochalcones
  • Flavanones
  • Fluorescence polarization
  • Mitochondrial outer membrane
  • Quercetin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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