Comparison of the effects of a bufodienolide and ouabain on neuronal and smooth muscle preparations

F. Oberfrank, E. Vízi, P. F. Baker, S. Samuelov, D. Lichtstein

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effect of a bufodienolide (monohydroxy-14,15-epoxy-20,22-dienolide glycoside) purified from toad skin was compared with that of ouabain on 3H-noradrenaline release and on the tension of rabbit pulmonary arterial strips. This compound exerted an ouabain-like activity. The neuronal effects of this bufodienolide derivative on squid axon were also studied and compared with those of ouabain. Both compounds enhanced the resting and stimulation-evoked (2 Hz. 360 shocks) release of 3H-noradrenaline. Moreover, in the presence of either this bufodienolide or ouabain, the tension of the rabbit artery increased gradually, and the contraction evoked by electrical stimulation was potentiated. Both compounds enhanced, in a prazosin-sensitive way, smooth muscle responses to noradrenaline and to electrical stimulation. In higher concentrations, they contracted smooth muscle cells of pulmonary artery, an action which was insensitive to prazosin. The bufodienolide was about 8 times more active in inhibition of 22Na efflux than was ouabain, but did not affect Ca efflux, which is not sensitive to ouabain. It is therefore concluded that compounds with an inhibitory effect on Na+,K+-ATPase are able to affect chemical neurotransmission of blood vessels in such a way that in lower concentrations they potentiate the release of noradrenaline, and in higher concentrations they contract directly the smooth muscle. These findings indicate that such compounds if they are present in the circulation might be involved in the physiological regulation of blood pressure or in the genesis of hypertension.

Original languageEnglish
Pages (from-to)235-244
Number of pages10
JournalNeuroscience Research
Volume10
Issue number4
DOIs
Publication statusPublished - 1991

Fingerprint

Ouabain
Smooth Muscle
Norepinephrine
Prazosin
Electric Stimulation
Rabbits
Decapodiformes
Glycosides
Synaptic Transmission
Anura
Pulmonary Artery
Smooth Muscle Myocytes
Blood Vessels
Axons
Shock
Arteries
Blood Pressure
Hypertension
Lung
Skin

Keywords

  • Blood vessel
  • Bufodienolide
  • Chemical neurotransmission
  • Endogenous inhibitors
  • Na,K-ATPase inhibition
  • Noradrenaline release

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Comparison of the effects of a bufodienolide and ouabain on neuronal and smooth muscle preparations. / Oberfrank, F.; Vízi, E.; Baker, P. F.; Samuelov, S.; Lichtstein, D.

In: Neuroscience Research, Vol. 10, No. 4, 1991, p. 235-244.

Research output: Contribution to journalArticle

Oberfrank, F. ; Vízi, E. ; Baker, P. F. ; Samuelov, S. ; Lichtstein, D. / Comparison of the effects of a bufodienolide and ouabain on neuronal and smooth muscle preparations. In: Neuroscience Research. 1991 ; Vol. 10, No. 4. pp. 235-244.
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