Comparison of the effect of hormones on the hormone synthesis of Tetrahymena in medium or salt solution

G. Csaba, Eszter Lajkó, Éva Pállinger

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8 Citations (Scopus)

Abstract

Tetrahymena pyriformis was maintained in TYM (tryptone-yeast medium) as well as in Losina salt solution. One hour treatment of 10-15 M histamine, serotonin or insulin was given before the histamine, serotonin, triiodothyronine and adrenocorticotropin contents of the cells were measured by flow cytometry after immunocytochemical staining. Maintenance in salt solution increased the hormone level in the cells, and use of the treatment hormone treatments further increased the endogenous hormone content relative to that in medium. The cells in salt mimic better the natural conditions, which means that the effects of hormones under more natural conditions are expressed to a greater extent than the exogenously given hormones in TYM typically used under laboratory conditions. Intercellular hormonal communication between the cells of a Tetrahymena population might assist in the survival of the individual cells.

Original languageEnglish
Pages (from-to)1095-1098
Number of pages4
JournalCell biology international
Volume34
Issue number11
DOIs
Publication statusPublished - Nov 1 2010

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Keywords

  • Hormonal effect
  • Hormone production
  • Medium
  • Milieu
  • Protozoan

ASJC Scopus subject areas

  • Cell Biology

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