Comparison of stress-induced PRINS gene expression in normal human keratinocytes and HaCaT cells

Lilla Bari, Sarolta Bacsa, Eniko Sonkoly, Z. Bata-Csörgő, L. Kemény, A. Dobozy, M. Széll

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Psoriasis is a chronic inflammatory skin disease that affects approximately 2-4% of the population. We recently described a novel non-coding RNA, psoriasis susceptibility related RNA gene induced by stress (PRINS), that was overexpressed in non-lesional psoriatic epidermis, and its expression was induced by various stress factors such as serum starvation, contact inhibition, ultraviolet (UV)-B irradiation, viral infection and translational inhibition in HaCaT cells. In the present work we set out to compare the stress and microbial agent-induced PRINS expression in normal human keratinocytes (NHKs) and HaCaT cells. Since nuclear factor-κB (NF-κB) is involved in the cellular stress response, we sought to explore whether there is a connection between the NF-κB and PRINS-mediated signal transduction pathways in NHKs and HaCaT cells. We found that the PRINS expression responded differentially to various stress signals and microbial agents in HaCaT cells and in NHKs: after translational inhibition and UV-B treatment, similar induction of PRINS expression occurred with different time courses while after microbial agent treatment, the PRINS expression was significantly induced in HaCaT cells, whereas we could not detect similar changes in NHKs. To explore whether the known NF-κB abnormalities in HaCaT cells could be related to this differential PRINS expression, we silenced the PRINS gene expression with small interfering RNA (siRNA) in both HaCaT cells and in NHKs and monitored NF-κB signal transduction after lipopolysaccharide (LPS) treatment. Silencing of PRINS had no effect on LPS-induced NF-κB activity either in HaCaT cells or in NHKs. Our results indicate that PRINS probably affects keratinocytes functions independently of NF-κB signalling.

Original languageEnglish
Pages (from-to)745-752
Number of pages8
JournalArchives of Dermatological Research
Volume303
Issue number10
DOIs
Publication statusPublished - Dec 2011

Fingerprint

Primed In Situ Labeling
Keratinocytes
Gene Expression
Psoriasis
Lipopolysaccharides
Signal Transduction
Contact Inhibition
Untranslated RNA
Virus Diseases
Starvation
Skin Diseases
Epidermis
Small Interfering RNA

Keywords

  • HaCaT cells
  • NF-κB
  • Non-coding RNA
  • Normal human keratinocytes
  • PRINS expression
  • Psoriasis

ASJC Scopus subject areas

  • Dermatology

Cite this

Comparison of stress-induced PRINS gene expression in normal human keratinocytes and HaCaT cells. / Bari, Lilla; Bacsa, Sarolta; Sonkoly, Eniko; Bata-Csörgő, Z.; Kemény, L.; Dobozy, A.; Széll, M.

In: Archives of Dermatological Research, Vol. 303, No. 10, 12.2011, p. 745-752.

Research output: Contribution to journalArticle

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