Comparison of separation performances of amylose- and cellulose-based stationary phases in the high-performance liquid chromatographic enantioseparation of stereoisomers of β-lactams

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

High-performance liquid chromatographic methods were developed for the separation of the enantiomers of 19 β-lactams. The direct separations were performed on chiral stationary phases containing either amylose-tris-3,5- dimethylphenyl carbamate, (Kromasil® AmyCoat™ column) or cellulose-tris-3,5-dimethylphenyl carbamate, (Kromasil® CelluCoat™ column) as chiral selector. The different methods were compared in systematic chromatographic examinations. The separations were carried out with good selectivity and resolution. The AmyCoat™ and CelluCoat™ columns appear to be highly complementary. The best separations of bi- and tricyclic b-lactam stereoisomers were obtained with the AmyCoat™ column, whereas the 4-aryl-substituted β-lactams were better separated on the CelluCoat™ column. The elution sequence was determined in all cases; no general rule could be established.

Original languageEnglish
Pages (from-to)120-128
Number of pages9
JournalChirality
Volume22
Issue number1
DOIs
Publication statusPublished - Jan 1 2010

    Fingerprint

Keywords

  • AmyCoat™ column
  • CelluCoat™ Column
  • Column liquid chromatography
  • β-lactams

ASJC Scopus subject areas

  • Analytical Chemistry
  • Catalysis
  • Pharmacology
  • Drug Discovery
  • Spectroscopy
  • Organic Chemistry

Cite this