Comparison of separation performances of amylose- and cellulose-based stationary phases in the high-performance liquid chromatographic enantioseparation of stereoisomers of β-lactams

Research output: Contribution to journalConference article

Abstract

High-performance liquid chromatographic methods were developed for the separation of the enantiomers of 19 β-lactams. The direct separations were performed on chiral stationary phases containing either amylose tris(3,5-dimethylphenylcarbamate), (Kromasil® AmyCoat™ column) or cellulose tris(3,5-dimethylphenylcarbamate), (Kromasil® CelluCoat™ column) as chiral selector. The different methods were compared in systematic chromatographic examinations. The separations were carried out with good selectivity and resolution. The AmyCoat™ and CelluCoat™ columns appear to be highly complementary. The best separations of bi- and tricyclic β-lactam stereoisomers were obtained with the AmyCoat™ column, while the 4-aryl-substituted β-lactams were better separated on the CelluCoat™ column. The elution sequence was determined in all cases; no general rule could be established.

Original languageEnglish
Pages (from-to)116-119
Number of pages4
JournalProcedia Chemistry
Volume2
Issue number1
DOIs
Publication statusPublished - Jan 1 2010
Event5th Symposium by Nordic Separation Science Society, NoSSS 2009 - Tallinn, Estonia
Duration: Aug 26 2009Aug 29 2009

Keywords

  • AmyCoat™ column
  • CelluCoat™ column
  • Column liquid chromatography
  • β-lactams

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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