Comparison of selective media for the isolation of Rhodococcus equi and description of a new selective plating medium

L. Makrai, L. Fodor, I. Vendég, G. Szigeti, B. Dénes, J. Reiczigel, J. Varga

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In order to improve the isolation rate of Rhodococcus equi from animals and soil, the efficacy of four previously described selective media (CAZ-NB, M3T, NANAT and TINSDALE) and that of four other media (NC, PNP, TCP and TVP) composed by us was compared and evaluated. Two selective plating media proved to be the best for the isolation of R. equi from contaminated samples. One of them was CAZ-NB containing ceftazidime, novobiocin and cycloheximide, while the other was the newly composed TCP containing trimethoprim, cefoperazone, polymyxin B, cycloheximide and potassium tellurite as selective components. These two media allowed the growth of at least 62-72% of R. equi present in the artificially contaminated samples, and the inhibition of unwanted contaminant bacteria and fungi was satisfactory with both media. TCP medium proved to be superior to CAZ-NB since the colony morphology of R. equi was much more characteristic (shiny, smooth, black colonies 3-5 mm in diameter) on it, and it inhibited the unwanted contaminant bacterial and fungal flora more effectively, especially in the case of faecal and soil samples. Therefore, TCP is recommended as a new, highly selective plating medium for the isolation of R. equi from contaminated samples.

Original languageEnglish
Pages (from-to)275-285
Number of pages11
JournalActa Veterinaria Hungarica
Volume53
Issue number3
DOIs
Publication statusPublished - 2005

Fingerprint

Rhodococcus equi
selective media
cycloheximide
Cycloheximide
Soil
cefoperazone
Novobiocin
polymyxin B
Cefoperazone
novobiocin
Polymyxin B
Trimethoprim
Ceftazidime
bacterial contamination
trimethoprim
sampling
Fungi
soil sampling
culture media
potassium

Keywords

  • Contaminated samples
  • Isolation
  • New selective plating medium
  • Rhodococcus equi
  • TCP

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{0d17953117e64b398916748926298a30,
title = "Comparison of selective media for the isolation of Rhodococcus equi and description of a new selective plating medium",
abstract = "In order to improve the isolation rate of Rhodococcus equi from animals and soil, the efficacy of four previously described selective media (CAZ-NB, M3T, NANAT and TINSDALE) and that of four other media (NC, PNP, TCP and TVP) composed by us was compared and evaluated. Two selective plating media proved to be the best for the isolation of R. equi from contaminated samples. One of them was CAZ-NB containing ceftazidime, novobiocin and cycloheximide, while the other was the newly composed TCP containing trimethoprim, cefoperazone, polymyxin B, cycloheximide and potassium tellurite as selective components. These two media allowed the growth of at least 62-72{\%} of R. equi present in the artificially contaminated samples, and the inhibition of unwanted contaminant bacteria and fungi was satisfactory with both media. TCP medium proved to be superior to CAZ-NB since the colony morphology of R. equi was much more characteristic (shiny, smooth, black colonies 3-5 mm in diameter) on it, and it inhibited the unwanted contaminant bacterial and fungal flora more effectively, especially in the case of faecal and soil samples. Therefore, TCP is recommended as a new, highly selective plating medium for the isolation of R. equi from contaminated samples.",
keywords = "Contaminated samples, Isolation, New selective plating medium, Rhodococcus equi, TCP",
author = "L. Makrai and L. Fodor and I. Vend{\'e}g and G. Szigeti and B. D{\'e}nes and J. Reiczigel and J. Varga",
year = "2005",
doi = "10.1556/AVet.53.2005.3.1",
language = "English",
volume = "53",
pages = "275--285",
journal = "Acta Veterinaria Hungarica",
issn = "0236-6290",
publisher = "Akademiai Kiado",
number = "3",

}

TY - JOUR

T1 - Comparison of selective media for the isolation of Rhodococcus equi and description of a new selective plating medium

AU - Makrai, L.

AU - Fodor, L.

AU - Vendég, I.

AU - Szigeti, G.

AU - Dénes, B.

AU - Reiczigel, J.

AU - Varga, J.

PY - 2005

Y1 - 2005

N2 - In order to improve the isolation rate of Rhodococcus equi from animals and soil, the efficacy of four previously described selective media (CAZ-NB, M3T, NANAT and TINSDALE) and that of four other media (NC, PNP, TCP and TVP) composed by us was compared and evaluated. Two selective plating media proved to be the best for the isolation of R. equi from contaminated samples. One of them was CAZ-NB containing ceftazidime, novobiocin and cycloheximide, while the other was the newly composed TCP containing trimethoprim, cefoperazone, polymyxin B, cycloheximide and potassium tellurite as selective components. These two media allowed the growth of at least 62-72% of R. equi present in the artificially contaminated samples, and the inhibition of unwanted contaminant bacteria and fungi was satisfactory with both media. TCP medium proved to be superior to CAZ-NB since the colony morphology of R. equi was much more characteristic (shiny, smooth, black colonies 3-5 mm in diameter) on it, and it inhibited the unwanted contaminant bacterial and fungal flora more effectively, especially in the case of faecal and soil samples. Therefore, TCP is recommended as a new, highly selective plating medium for the isolation of R. equi from contaminated samples.

AB - In order to improve the isolation rate of Rhodococcus equi from animals and soil, the efficacy of four previously described selective media (CAZ-NB, M3T, NANAT and TINSDALE) and that of four other media (NC, PNP, TCP and TVP) composed by us was compared and evaluated. Two selective plating media proved to be the best for the isolation of R. equi from contaminated samples. One of them was CAZ-NB containing ceftazidime, novobiocin and cycloheximide, while the other was the newly composed TCP containing trimethoprim, cefoperazone, polymyxin B, cycloheximide and potassium tellurite as selective components. These two media allowed the growth of at least 62-72% of R. equi present in the artificially contaminated samples, and the inhibition of unwanted contaminant bacteria and fungi was satisfactory with both media. TCP medium proved to be superior to CAZ-NB since the colony morphology of R. equi was much more characteristic (shiny, smooth, black colonies 3-5 mm in diameter) on it, and it inhibited the unwanted contaminant bacterial and fungal flora more effectively, especially in the case of faecal and soil samples. Therefore, TCP is recommended as a new, highly selective plating medium for the isolation of R. equi from contaminated samples.

KW - Contaminated samples

KW - Isolation

KW - New selective plating medium

KW - Rhodococcus equi

KW - TCP

UR - http://www.scopus.com/inward/record.url?scp=23844526487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23844526487&partnerID=8YFLogxK

U2 - 10.1556/AVet.53.2005.3.1

DO - 10.1556/AVet.53.2005.3.1

M3 - Article

VL - 53

SP - 275

EP - 285

JO - Acta Veterinaria Hungarica

JF - Acta Veterinaria Hungarica

SN - 0236-6290

IS - 3

ER -