Comparison of Selected Stationary Phases for Determination of Vancomycin and Ciprofloxacin Using Buffered Mobile Phases, with and Without Triethylamine

P. Forlay-Frick, J. Fekete

Research output: Contribution to journalArticle

6 Citations (Scopus)


In our study we measured the retention factors, the theoretical plate numbers, and the symmetry factors in the case of phosphate buffer and triethylamine containing phosphate buffer, in order to get some information about the surface characteristic of tested columns. Three solutes (N,N-dimethyl-aniline, Ciprofloxacin, and Vancomycin) and 7 stationary phases (Nucleosil® 100-C-8, LiChrosorb® RP-select B, LiChrospher® RP-18, Purospher® RP-18e, Prontosil® 120-5-C18-AQ, Symmetry® C-18, and Chromolith® SpeedROD RP-18e) were used in this study. One of the solutes, N,N-dimethyl-aniline (121 g/mol) is a basic, low molecular weight compound. This is widely used for characterization of silanol activity of chemically bonded phases. The other two, Ciprofloxacin (331 g/mol) and Vancomycin (1449 g/mol), contain both basic and acidic groups, and Vancomycin has a high molecular weight. These latter two are real life samples and we tried to find a correlation between results of test compounds and these widely used antibiotics. For this purpose, we selected widely different columns: end-capped phases, non end-capped phases with high and low metal content, and last but not least, a recently developed monolithic silica column. According to selection criteria (N/m > 20,000; 0.8 < As < 1.8), we evaluated the effect of masking agent (triethylamine) and the role of organic solvents (methanol and acetonitrile). It was found that the Symmetry C-18 was the best in the case of Ciprofloxacin and N,N-dimethyl-aniline, and the Chromolith SpeedROD RP-18e monolithic silica rod in the case of high molecular weight Vancomycin in these experiments. In most of the cases the LiChrosorb RP-select B had to be excluded. Based on selection criteria, the eluent composition and the structure and size of tested solutes strongly influenced the applicability of other columns. Influence of TEA on theoretical plate number and symmetry factor was very high in the case of these columns.

Original languageEnglish
Pages (from-to)123-143
Number of pages21
JournalJournal of Liquid Chromatography and Related Technologies
Issue number1
Publication statusPublished - Mar 5 2004



  • Buffer
  • Ciprofloxacin
  • RP stationary phases
  • Triethylamine
  • Vancomycin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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