Comparison of roles of serine esterase in chicken and human natural cytotoxicity

Yvette Mándi, Salem Ibrahim Garssadi, Ilona Béládi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The requirements for serine esterase activity in the spontaneous cell-mediated cytotoxicity of human lymphocytes and chicken granulocytes have been compared. The lysis of K-562 target cells and of LSCC-H32 chicken target cells was prevented by the serine esterase inhibitor TPCK. ATEE, the substrate of chymotrypsin, impaired both cytotoxic reactions, but to a lesser degree the cytotoxicity of chicken granulocytes. TPCK inhibited the "trigger" mechanism in an early calcium-dependent step and later calcium-independent events in both systems. However, calcium-independent lysis was depressed by serine esterase inhibitor only in the avian cytotoxicity. These findings suggest that avian target cell cytolysis consists of similar sequential phases to those already demonstrated in the human NK cell reaction, and serine esterase is required during several stages of cytotoxicity in the avian system.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalDevelopmental and Comparative Immunology
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 1 1990

Keywords

  • Chicken granulocytes
  • Human NK cells
  • Natural cytotoxicity
  • Serine esterase

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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