Comparison of RFLP probes and RAPD primers for studying genetic diversity in barley (Hordeum vulgare L.)

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Abstract

The major aim of this experiment was to compare the effectivness of RFLP probes and RAPD primers in evaluating genetic diversity and separating different groups of barley (Hordeum vulgare L.). For this purpose, five barley varieties were chosen to represent different groups based on growth habit and head type. They were tested parallel with 12 RFLP probes in five enzyme combinations and with 19 RAPD primers. The number of DNA products and the frequency of the polymorphic bands generated by RAPD primers were higher than those obtained with the RFLP probes. The value of the polymorphic bands averaged over all the variety x primer or all the variety x probe x enzyme combinations was 3.50 for the RAPD primers and 0.82 for the RFLP probes. On the other band RFLP was more informative as there was much lower variation in the estimations of genetic diversity using the individual RFLP probes than using the individual RAPD primers. There was a definite distinction between the different groups of barley with RFLP, while only the variety with two- rowed heads was separated from the others with RAPD. The correlation between the JD genetic diversity values generated by the two methods was r2=0.69. Based on the results. RFLP probes appear more efficient in establishing a reliable grouping of relatedness between varieties, while RAPD primers are more effective than the RFLP probes in giving a good characterisation of individual varieties and breeding materials.

Original languageEnglish
Pages (from-to)283-290
Number of pages8
JournalCereal Research Communications
Volume24
Issue number3
Publication statusPublished - 1996

Fingerprint

Hordeum
Restriction Fragment Length Polymorphisms
Hordeum vulgare
restriction fragment length polymorphism
barley
genetic variation
Head
growth habit
Enzymes
enzymes
Habits
Breeding
DNA
breeding
Growth

Keywords

  • barley
  • genetic diversity
  • RAPD
  • RFLP

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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abstract = "The major aim of this experiment was to compare the effectivness of RFLP probes and RAPD primers in evaluating genetic diversity and separating different groups of barley (Hordeum vulgare L.). For this purpose, five barley varieties were chosen to represent different groups based on growth habit and head type. They were tested parallel with 12 RFLP probes in five enzyme combinations and with 19 RAPD primers. The number of DNA products and the frequency of the polymorphic bands generated by RAPD primers were higher than those obtained with the RFLP probes. The value of the polymorphic bands averaged over all the variety x primer or all the variety x probe x enzyme combinations was 3.50 for the RAPD primers and 0.82 for the RFLP probes. On the other band RFLP was more informative as there was much lower variation in the estimations of genetic diversity using the individual RFLP probes than using the individual RAPD primers. There was a definite distinction between the different groups of barley with RFLP, while only the variety with two- rowed heads was separated from the others with RAPD. The correlation between the JD genetic diversity values generated by the two methods was r2=0.69. Based on the results. RFLP probes appear more efficient in establishing a reliable grouping of relatedness between varieties, while RAPD primers are more effective than the RFLP probes in giving a good characterisation of individual varieties and breeding materials.",
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T1 - Comparison of RFLP probes and RAPD primers for studying genetic diversity in barley (Hordeum vulgare L.)

AU - Linc, G.

AU - Karsai, I.

AU - Molnár-Láng, M.

AU - Bedő, Z.

PY - 1996

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N2 - The major aim of this experiment was to compare the effectivness of RFLP probes and RAPD primers in evaluating genetic diversity and separating different groups of barley (Hordeum vulgare L.). For this purpose, five barley varieties were chosen to represent different groups based on growth habit and head type. They were tested parallel with 12 RFLP probes in five enzyme combinations and with 19 RAPD primers. The number of DNA products and the frequency of the polymorphic bands generated by RAPD primers were higher than those obtained with the RFLP probes. The value of the polymorphic bands averaged over all the variety x primer or all the variety x probe x enzyme combinations was 3.50 for the RAPD primers and 0.82 for the RFLP probes. On the other band RFLP was more informative as there was much lower variation in the estimations of genetic diversity using the individual RFLP probes than using the individual RAPD primers. There was a definite distinction between the different groups of barley with RFLP, while only the variety with two- rowed heads was separated from the others with RAPD. The correlation between the JD genetic diversity values generated by the two methods was r2=0.69. Based on the results. RFLP probes appear more efficient in establishing a reliable grouping of relatedness between varieties, while RAPD primers are more effective than the RFLP probes in giving a good characterisation of individual varieties and breeding materials.

AB - The major aim of this experiment was to compare the effectivness of RFLP probes and RAPD primers in evaluating genetic diversity and separating different groups of barley (Hordeum vulgare L.). For this purpose, five barley varieties were chosen to represent different groups based on growth habit and head type. They were tested parallel with 12 RFLP probes in five enzyme combinations and with 19 RAPD primers. The number of DNA products and the frequency of the polymorphic bands generated by RAPD primers were higher than those obtained with the RFLP probes. The value of the polymorphic bands averaged over all the variety x primer or all the variety x probe x enzyme combinations was 3.50 for the RAPD primers and 0.82 for the RFLP probes. On the other band RFLP was more informative as there was much lower variation in the estimations of genetic diversity using the individual RFLP probes than using the individual RAPD primers. There was a definite distinction between the different groups of barley with RFLP, while only the variety with two- rowed heads was separated from the others with RAPD. The correlation between the JD genetic diversity values generated by the two methods was r2=0.69. Based on the results. RFLP probes appear more efficient in establishing a reliable grouping of relatedness between varieties, while RAPD primers are more effective than the RFLP probes in giving a good characterisation of individual varieties and breeding materials.

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