Comparison of mannitol and xylitol as osmolytes in preparation of mitochondria

B. Tomasskovics, A. Szarka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

Mitochondrial research is performed on mitochondria prepared from different animal and plant tissues. The preparation of intact mitochondria is a prerequisite for the successful research. The osmoticum in the basic homogenization medium maintains the mitochondrial structure and prevents physical swelling and rupture of membranes. Thus the six-carbon mannitol and the five-carbon xylitol was compared both in the preparation of animal and plant mitochondria to find a possible new non six-carbon osmolyte. The intactness of the isolated mitochondria reached high degree from both sources, if mannitol was applied as the osmolyte, however the intactness was low in the case of xylitol osmolite. On the base of this result the six-carbon mannitol is a better osmolyte, than the five-carbon xylitol.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages1362-1365
Number of pages4
Volume37
DOIs
Publication statusPublished - 2011

Fingerprint

Xylitol
Mitochondria
Mannitol
Carbon
Animals
Swelling
Tissue
Membranes

Keywords

  • mannitol
  • mitochondria isolation
  • osmolyte
  • xylitol

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Comparison of mannitol and xylitol as osmolytes in preparation of mitochondria. / Tomasskovics, B.; Szarka, A.

IFMBE Proceedings. Vol. 37 2011. p. 1362-1365.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tomasskovics, B. ; Szarka, A. / Comparison of mannitol and xylitol as osmolytes in preparation of mitochondria. IFMBE Proceedings. Vol. 37 2011. pp. 1362-1365
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