Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction

D. Djikanović, A. Devečerski, G. Steinbach, J. Simonović, B. Matović, G. Garab, A. Kalauzi, K. Radotić

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Interactions between macromolecules in the cell walls of different plant origin were compared, namely spruce wood (Picea omorika (Pančić) Purkiňe) as an example of softwood, maple wood (Acer platanoides L.) as a hardwood and maize stems (Zea mays L.) as a herbaceous plant from the grass family and widely used agricultural plant. Interactions of macromolecules in isolated cell walls from the three species were compared by using Fourier transform infrared spectroscopy, X-ray diffraction and fluorescence spectroscopy. Linear dichroism of the cell walls was observed by using differential polarization laser scanning microscope (DP-LSM), which provides information of macromolecular order. This method has not previously been used for comparison of the cell walls of various plant origins. It was shown that the maize cell walls have higher amount of hydrogen bonds that lead to more regular packing of cellulose molecules, simpler structure of lignin, and a higher crystallinity of the cell wall in relation to the walls of woody plants. DP-LSM and fluorescence spectroscopy results indicate that maize has simpler and more ordered structure than both woody species. The results of this work provide new data for comparison of the cell wall properties that may be important for selection of appropriate plant for possible applications as a source of biomass. This may be a contribution to the development of efficient deconstruction and separation technologies that enable release of sugar and aromatic compounds from the cell wall macromolecular structure.

Original languageEnglish
Pages (from-to)1-20
Number of pages20
JournalWood Science and Technology
DOIs
Publication statusAccepted/In press - Dec 8 2015

Fingerprint

fluorescence spectroscopy
Softwoods
Hardwoods
softwood
FTIR spectroscopy
X-ray diffraction
hardwood
lasers
microscopy
spectroscopy
Microscopic examination
polarization
laser
Fluorescence
maize
Cells
cell walls
Spectroscopy
fluorescence
Polarization

ASJC Scopus subject areas

  • Forestry
  • Plant Science
  • Materials Science(all)
  • Industrial and Manufacturing Engineering

Cite this

Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction. / Djikanović, D.; Devečerski, A.; Steinbach, G.; Simonović, J.; Matović, B.; Garab, G.; Kalauzi, A.; Radotić, K.

In: Wood Science and Technology, 08.12.2015, p. 1-20.

Research output: Contribution to journalArticle

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