Comparison of lectin induced chemotactic selection and chemical imprinting in Tetrahymena pyriformis

L. Kőhidai, Csaba Bánky, G. Csaba

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Summary. In case of hormonal (chemical) imprinting, the first encounter of a mixed cell population with a bioactive molecule provokes changes in the receptor-signal transduction system. In case of chemotactic selection, from the mixed cell culture the chemotactically most affine cells are selected and maintained. In the present experiments chemotactic effects elicited by three lectins (Concanavalin-A = Con A, lens, Helix) were studied in Tetrahymena pyriformis GL unicellular model, for comparing the effect of imprinting and selection. Tetrahymena (without selection or imprinting) showed a molecule-dependent (positive) chemotactic reaction to lectins. Subpopulations gained by chemotactic selection were inducible in a ligand-specific scale with preference of [α-Man-α-Glu] or N-Ac-Gal specificity lectins, producing a significant affinity to Helix and non-significant affinity to Con A, and negative chemotaxis to lens. Similar results were shown in case of selection and imprinting with Con-A (except the significant affinity of mixed population), nevertheless in case of Helix and lens lectins (having similar, however not identical sugar specificity) differences had been observed between selection and imprinting. These accordances and deviations of results in imprinting and selection studies suggest that chemotaxis receptors share functional moieties with lectin receptors of unicellular organisms. The experiments call attention to the possible role of selection in the development of imprinting however, this is dependent on the nature of the imprinter molecule.

Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalActa Protozoologica
Volume42
Issue number2
Publication statusPublished - May 2003

Fingerprint

Tetrahymena pyriformis
Lectins
lectins
concanavalin A
Lens
chemotaxis
Lenses
receptors
Chemotaxis
Tetrahymena
Mitogen Receptors
signal transduction
cell culture
Concanavalin A
Imprinting (Psychology)
cells
sugars
Population
Signal Transduction
organisms

Keywords

  • Chemotactic selection
  • Chemotaxis
  • Hormonal imprinting
  • Lectin
  • Tetrahymena pyriformis GL

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)
  • Microbiology

Cite this

Comparison of lectin induced chemotactic selection and chemical imprinting in Tetrahymena pyriformis. / Kőhidai, L.; Bánky, Csaba; Csaba, G.

In: Acta Protozoologica, Vol. 42, No. 2, 05.2003, p. 91-97.

Research output: Contribution to journalArticle

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