Comparison of laser-assisted hatching and acidified Tyrode's hatching by evaluation of blastocyst development rates in sibling embryos: A prospective randomized trial

Amy E. Jones, Graham Wright, Hilton I. Kort, Robert J. Straub, Zsolt P. Nagy

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Objective: To assess two zona drilling methods in terms of blastocyst development rates using sister embryos. Design: Prospective, randomized study. Sister embryos of 14 patients were randomly assigned on day 3 to acidified Tyrode's zona drilling or to laser zona drilling. After biopsy, subsequent embryo culture until the blastocyst stage (day 5) was performed. Setting: Private fertility center. Patient(s): Patients undergoing IVF-preimplantation genetic diagnosis. Intervention(s): Embryo biopsy using either laser-assisted hatching or acidified Tyrode's hatching on sibling embryos and subsequent blastocyst development evaluation. Main Outcome Measure(s): Evaluation of blastocyst development in terms of degree of expansion and cell number in the inner cell mass and trophectoderm. Result(s): Blastocyst development rates (and blastocyst quality) were similarly high in both the acidified Tyrode's hatching group and the laser-assisted hatching group. Conclusion(s): Laser hatching does not impair embryonic development to the blastocyst stage, demonstrating that laser-assisted hatching is a suitable alternative to the use of acidified Tyrode's solution for zona drilling.

Original languageEnglish
Pages (from-to)487-491
Number of pages5
JournalFertility and Sterility
Volume85
Issue number2
DOIs
Publication statusPublished - Feb 1 2006

Keywords

  • Acidified Tyrode's hatching
  • Blastocyst development
  • Laser-assisted hatching
  • Preimplantation genetic diagnosis

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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