Comparison of intestinal warm ischemic injury in PACAP knockout and wild-type mice

A. Ferencz, P. Kiss, G. Wéber, Z. Helyes, Norihito Shintani, Akemichi Baba, D. Reglodi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is present in the gastrointestinal tract and plays a central role in the intestinal physiology, mainly in the secretion and motility. The aim of our study was to compare the ischemic injury in wild-type and PACAP-38 knockout mice following warm mesenteric small bowel ischemia. Warm ischemia groups were designed with occlusion of superior mesenteric artery for 1, 3, and 6 h in wild-type (n∈=∈10 in each group) and PACAP-38 knockout (n∈=∈10 in each group) mice. Small bowel biopsies were collected after laparotomy (control) and at the end of the ischemia periods. To determine oxidative stress parameters, malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD) were measured. Tissue damage was analyzed by qualitative and quantitative methods on hematoxylin/eosin-stained sections. In PACAP-38 knockout animals, tissue MDA increased significantly after 3 and 6 h ischemia (133. 97∈±∈6,2; 141.86∈±∈5,8) compared to sham-operated (100.92∈±∈3,6) and compared to wild-type results (112.8∈±∈2,1; 118.4∈±∈1.03 μmol/g, p∈

Original languageEnglish
Pages (from-to)435-442
Number of pages8
JournalJournal of Molecular Neuroscience
Volume42
Issue number3
DOIs
Publication statusPublished - Nov 2010

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Knockout Mice
Ischemia
Wounds and Injuries
Malondialdehyde
Warm Ischemia
Superior Mesenteric Artery
Hematoxylin
Eosine Yellowish-(YS)
Laparotomy
Superoxide Dismutase
Glutathione
Gastrointestinal Tract
Oxidative Stress
Biopsy

Keywords

  • Oxidative stress
  • PACAP-38
  • Small bowel
  • Tissue injury
  • Warm ischemia

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Comparison of intestinal warm ischemic injury in PACAP knockout and wild-type mice. / Ferencz, A.; Kiss, P.; Wéber, G.; Helyes, Z.; Shintani, Norihito; Baba, Akemichi; Reglodi, D.

In: Journal of Molecular Neuroscience, Vol. 42, No. 3, 11.2010, p. 435-442.

Research output: Contribution to journalArticle

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