Comparison of ethylene glycol and propylene glycol for the vitrification of immature porcine oocytes

Tamás Somfai, Michiko Nakai, Fuminori Tanihara, Junko Noguchi, Hiroyuki Kaneko, Naomi Kashiwazaki, I. Egerszegi, Takashi Nagai, Kazuhiro Kikuchi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Our aim was to optimize a cryoprotectant treatment for vitrification of immature porcine cumulus-oocyte complexes (COCs). Immature COCs were vitrified either in 35% ethylene glycol (EG), 35% propylene glycol (PG) or a combination of 17.5% EG and 17.5% PG. After warming, the COCs were in vitro matured (IVM), and surviving oocytes were in vitro fertilized (IVF) and cultured. The mean survival rate of vitrified oocytes in 35% PG (73.9%) was higher (P

Original languageEnglish
Pages (from-to)378-384
Number of pages7
JournalJournal of Reproduction and Development
Volume59
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

propylene glycol
ethylene glycol
vitrification
oocytes
immatures
swine
cryoprotectants
survival rate

Keywords

  • Cryoprotectant
  • Embryo development
  • Immature oocyte
  • In vitro fertilization
  • Pig
  • Vitrification

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Somfai, T., Nakai, M., Tanihara, F., Noguchi, J., Kaneko, H., Kashiwazaki, N., ... Kikuchi, K. (2013). Comparison of ethylene glycol and propylene glycol for the vitrification of immature porcine oocytes. Journal of Reproduction and Development, 59(4), 378-384. https://doi.org/10.1262/jrd.2013-015

Comparison of ethylene glycol and propylene glycol for the vitrification of immature porcine oocytes. / Somfai, Tamás; Nakai, Michiko; Tanihara, Fuminori; Noguchi, Junko; Kaneko, Hiroyuki; Kashiwazaki, Naomi; Egerszegi, I.; Nagai, Takashi; Kikuchi, Kazuhiro.

In: Journal of Reproduction and Development, Vol. 59, No. 4, 2013, p. 378-384.

Research output: Contribution to journalArticle

Somfai, T, Nakai, M, Tanihara, F, Noguchi, J, Kaneko, H, Kashiwazaki, N, Egerszegi, I, Nagai, T & Kikuchi, K 2013, 'Comparison of ethylene glycol and propylene glycol for the vitrification of immature porcine oocytes', Journal of Reproduction and Development, vol. 59, no. 4, pp. 378-384. https://doi.org/10.1262/jrd.2013-015
Somfai, Tamás ; Nakai, Michiko ; Tanihara, Fuminori ; Noguchi, Junko ; Kaneko, Hiroyuki ; Kashiwazaki, Naomi ; Egerszegi, I. ; Nagai, Takashi ; Kikuchi, Kazuhiro. / Comparison of ethylene glycol and propylene glycol for the vitrification of immature porcine oocytes. In: Journal of Reproduction and Development. 2013 ; Vol. 59, No. 4. pp. 378-384.
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