Comparison of early onco/suppressor gene expressions in peripheral leukocytes and potential target organs of rats exposed to the carcinogen 1-nitropyrene

I. Ember, I. Kiss, Z. Gyöngyi, Cs Varga

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An in-vivo model has been developed to study early expressions of c-myc, Ha-ras oncogenes and p53 suppressor gene as biomarkers of carcinogenic exposure and/or tumorigenesis. In order to validate the in-vivo expression changes as biomarkers, rats were treated with the outdoor air pollutant carcinogen 1-nitropyrene. The gene expression levels were measured after 24 and 48 h in potential target tissues (lung, liver, lymph nodes, kidneys, spleen) and in peripheral blood leukocytes. Another main objective was to prove the applicability of leukocytes as a surrogate tissue, having a similar expression pattern of the selected genes upon carcinogenic exposure. The c-myc oncogene was not suitable as an early biomarker because of the lack or low level of its expression. However, in the case of the other oncogene Ha-ras and the suppressor gene p53, remarkable and early changes were detected in the expression signals. Similar expression patterns could only be detected in leukocytes and the spleen; therefore we continue this validation study by using other types and routes of exposure.

Original languageEnglish
Pages (from-to)439-442
Number of pages4
JournalEuropean Journal of Cancer Prevention
Volume9
Issue number6
DOIs
Publication statusPublished - 2000

Fingerprint

1-nitropyrene
Tumor Suppressor Genes
Carcinogens
Suppressor Genes
Leukocytes
ras Genes
Biomarkers
Gene Expression
Spleen
Air Pollutants
myc Genes
Validation Studies
p53 Genes
Oncogenes
Carcinogenesis
Lymph Nodes
Kidney
Lung
Liver
Genes

Keywords

  • 1-nitropyrene exposure
  • C-myc
  • Gene expression
  • Ha-ras
  • Leukocytes
  • Long-Evans rat
  • Oncogene
  • p53
  • Suppressor gene
  • Surrogate biomarker

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

@article{1e196d2125444bea82c037389057e325,
title = "Comparison of early onco/suppressor gene expressions in peripheral leukocytes and potential target organs of rats exposed to the carcinogen 1-nitropyrene",
abstract = "An in-vivo model has been developed to study early expressions of c-myc, Ha-ras oncogenes and p53 suppressor gene as biomarkers of carcinogenic exposure and/or tumorigenesis. In order to validate the in-vivo expression changes as biomarkers, rats were treated with the outdoor air pollutant carcinogen 1-nitropyrene. The gene expression levels were measured after 24 and 48 h in potential target tissues (lung, liver, lymph nodes, kidneys, spleen) and in peripheral blood leukocytes. Another main objective was to prove the applicability of leukocytes as a surrogate tissue, having a similar expression pattern of the selected genes upon carcinogenic exposure. The c-myc oncogene was not suitable as an early biomarker because of the lack or low level of its expression. However, in the case of the other oncogene Ha-ras and the suppressor gene p53, remarkable and early changes were detected in the expression signals. Similar expression patterns could only be detected in leukocytes and the spleen; therefore we continue this validation study by using other types and routes of exposure.",
keywords = "1-nitropyrene exposure, C-myc, Gene expression, Ha-ras, Leukocytes, Long-Evans rat, Oncogene, p53, Suppressor gene, Surrogate biomarker",
author = "I. Ember and I. Kiss and Z. Gy{\"o}ngyi and Cs Varga",
year = "2000",
doi = "10.1097/00008469-200012000-00011",
language = "English",
volume = "9",
pages = "439--442",
journal = "European Journal of Cancer Prevention",
issn = "0959-8278",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Comparison of early onco/suppressor gene expressions in peripheral leukocytes and potential target organs of rats exposed to the carcinogen 1-nitropyrene

AU - Ember, I.

AU - Kiss, I.

AU - Gyöngyi, Z.

AU - Varga, Cs

PY - 2000

Y1 - 2000

N2 - An in-vivo model has been developed to study early expressions of c-myc, Ha-ras oncogenes and p53 suppressor gene as biomarkers of carcinogenic exposure and/or tumorigenesis. In order to validate the in-vivo expression changes as biomarkers, rats were treated with the outdoor air pollutant carcinogen 1-nitropyrene. The gene expression levels were measured after 24 and 48 h in potential target tissues (lung, liver, lymph nodes, kidneys, spleen) and in peripheral blood leukocytes. Another main objective was to prove the applicability of leukocytes as a surrogate tissue, having a similar expression pattern of the selected genes upon carcinogenic exposure. The c-myc oncogene was not suitable as an early biomarker because of the lack or low level of its expression. However, in the case of the other oncogene Ha-ras and the suppressor gene p53, remarkable and early changes were detected in the expression signals. Similar expression patterns could only be detected in leukocytes and the spleen; therefore we continue this validation study by using other types and routes of exposure.

AB - An in-vivo model has been developed to study early expressions of c-myc, Ha-ras oncogenes and p53 suppressor gene as biomarkers of carcinogenic exposure and/or tumorigenesis. In order to validate the in-vivo expression changes as biomarkers, rats were treated with the outdoor air pollutant carcinogen 1-nitropyrene. The gene expression levels were measured after 24 and 48 h in potential target tissues (lung, liver, lymph nodes, kidneys, spleen) and in peripheral blood leukocytes. Another main objective was to prove the applicability of leukocytes as a surrogate tissue, having a similar expression pattern of the selected genes upon carcinogenic exposure. The c-myc oncogene was not suitable as an early biomarker because of the lack or low level of its expression. However, in the case of the other oncogene Ha-ras and the suppressor gene p53, remarkable and early changes were detected in the expression signals. Similar expression patterns could only be detected in leukocytes and the spleen; therefore we continue this validation study by using other types and routes of exposure.

KW - 1-nitropyrene exposure

KW - C-myc

KW - Gene expression

KW - Ha-ras

KW - Leukocytes

KW - Long-Evans rat

KW - Oncogene

KW - p53

KW - Suppressor gene

KW - Surrogate biomarker

UR - http://www.scopus.com/inward/record.url?scp=0034515061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034515061&partnerID=8YFLogxK

U2 - 10.1097/00008469-200012000-00011

DO - 10.1097/00008469-200012000-00011

M3 - Article

C2 - 11201684

AN - SCOPUS:0034515061

VL - 9

SP - 439

EP - 442

JO - European Journal of Cancer Prevention

JF - European Journal of Cancer Prevention

SN - 0959-8278

IS - 6

ER -