Comparison of early onco/suppressor gene expressions in peripheral leukocytes and potential target organs of rats exposed to the carcinogen 1-nitropyrene

I. Ember, I. Kiss, Z. Gyöngyi, Cs Varga

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An in-vivo model has been developed to study early expressions of c-myc, Ha-ras oncogenes and p53 suppressor gene as biomarkers of carcinogenic exposure and/or tumorigenesis. In order to validate the in-vivo expression changes as biomarkers, rats were treated with the outdoor air pollutant carcinogen 1-nitropyrene. The gene expression levels were measured after 24 and 48 h in potential target tissues (lung, liver, lymph nodes, kidneys, spleen) and in peripheral blood leukocytes. Another main objective was to prove the applicability of leukocytes as a surrogate tissue, having a similar expression pattern of the selected genes upon carcinogenic exposure. The c-myc oncogene was not suitable as an early biomarker because of the lack or low level of its expression. However, in the case of the other oncogene Ha-ras and the suppressor gene p53, remarkable and early changes were detected in the expression signals. Similar expression patterns could only be detected in leukocytes and the spleen; therefore we continue this validation study by using other types and routes of exposure.

Original languageEnglish
Pages (from-to)439-442
Number of pages4
JournalEuropean Journal of Cancer Prevention
Volume9
Issue number6
DOIs
Publication statusPublished - Dec 1 2000

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Keywords

  • 1-nitropyrene exposure
  • C-myc
  • Gene expression
  • Ha-ras
  • Leukocytes
  • Long-Evans rat
  • Oncogene
  • Suppressor gene
  • Surrogate biomarker
  • p53

ASJC Scopus subject areas

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

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