Comparison of different treatments for parthenogenetic activation of bovine oocytes matured in vitro

Sz Bodó, A. Dinnyés, B. Baranyai, L. Solti, J. Dohy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In vitro matured bovine oocytes were treated by three parthenogenetic activation methods. Basic treatment with Ca-ionophore and cytochalasin D was combined with cycloheximide (Group 2), electric impulse (Group 3), and cycloheximide and electric pulse (Group 1) treatments, respectively. Survival and the in vitro development of parthenogenetic embryos to hatched blastocyst stage were compared. Rates of the first cleavage and morula development significantly differed among the treatment groups. Further development rates of the treated embryos up to blastocyst stage did not differ. The best results were obtained by the combination of cycloheximide and electric treatments (Group 1; 14% blastocyst, 7% hatched blastocyst). The results suggest that the combined treatment for oocyte activation is the most efficient and should be applied for nuclear cloning procedures.

Original languageEnglish
Pages (from-to)493-500
Number of pages8
JournalActa Veterinaria Hungarica
Volume46
Issue number4
Publication statusPublished - 1998

Fingerprint

blastocyst
Oocytes
Blastocyst
cycloheximide
oocytes
Cycloheximide
cattle
electrical treatment
cytochalasin D
morula
ionophores
Therapeutics
Morula
Cytochalasin D
Ionophores
molecular cloning
embryo (animal)
embryogenesis
Embryonic Development
Organism Cloning

Keywords

  • Bovine
  • In vitro produced embryos
  • Oocyte activation
  • Parthenogenesis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Comparison of different treatments for parthenogenetic activation of bovine oocytes matured in vitro. / Bodó, Sz; Dinnyés, A.; Baranyai, B.; Solti, L.; Dohy, J.

In: Acta Veterinaria Hungarica, Vol. 46, No. 4, 1998, p. 493-500.

Research output: Contribution to journalArticle

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