Comparison of control analysis data using different approaches

Modelling and experiments with muscle extract

Joaquim Puigjaner, Badr Raïs, Montse Burgos, Begoña Comin, J. Ovádi, Marta Cascante

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Experimental and model studies have been performed to characterize the control properties of hexokinase and phosphofructokinase in muscle glycolysis and to examine the nature of error associated with experimental flux control coefficient determinations. Different approaches of metabolic control analysis, classical titration, co-response analysis and kinetic modelling indicated that flux control coefficients could be reliably estimated experimentally for the upper part of glycolysis. The kinetic parameters applied to construct the mathematical model were determined in muscle extract under similar conditions used for flux studies. If the kinetic parameters of commercial enzymes are introduced into the model the control analysis data cannot be trusted. Co-response analysis can also be successfully applied to determination of the flux control coefficients of the system. However, the involvement of a rapid-equilibrium enzyme, such as glucose 6-phosphate isomerase, could result in estimation errors for the relevant co-response coefficients that are propagated into the elasticity matrix. If the co-response coefficients related to isomerase activity are replaced by the values obtained by kinetic modelling, the values of elasticities are correct. Our data also suggest that in the upper part of glycolysis hexokinase mainly controls the pathway flux whereas phosphofructokinase exerts dominant control on the turnover of internal metabolite stocks inside the system.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalFEBS Letters
Volume418
Issue number1-2
DOIs
Publication statusPublished - Nov 24 1997

Fingerprint

Muscle
Glycolysis
Phosphofructokinases
Muscles
Hexokinase
Elasticity
Fluxes
Theoretical Models
Experiments
Glucose-6-Phosphate Isomerase
Isomerases
Enzymes
Kinetic parameters
Kinetics
Metabolites
Titration
Error analysis
Mathematical models

Keywords

  • Co-response analysis
  • Glycolysis
  • Metabolic control analysis
  • Modelling
  • Muscle

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Comparison of control analysis data using different approaches : Modelling and experiments with muscle extract. / Puigjaner, Joaquim; Raïs, Badr; Burgos, Montse; Comin, Begoña; Ovádi, J.; Cascante, Marta.

In: FEBS Letters, Vol. 418, No. 1-2, 24.11.1997, p. 47-52.

Research output: Contribution to journalArticle

Puigjaner, Joaquim ; Raïs, Badr ; Burgos, Montse ; Comin, Begoña ; Ovádi, J. ; Cascante, Marta. / Comparison of control analysis data using different approaches : Modelling and experiments with muscle extract. In: FEBS Letters. 1997 ; Vol. 418, No. 1-2. pp. 47-52.
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