Comparison of Circulating miRNAs Expression Alterations in Matched Tissue and Plasma Samples During Colorectal Cancer Progression

Zsófia Brigitta Nagy, Barbara Kinga Barták, Alexandra Kalmár, O. Galamb, Barnabás Wichmann, Magdolna Dank, P. Igaz, Z. Tulassay, B. Molnár

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) have been found to play a critical role in colorectal adenoma-carcinoma sequence. MiRNA-specific high-throughput arrays became available to detect promising miRNA expression alterations even in biological fluids, such as plasma samples, where miRNAs are stable. The purpose of this study was to identify circulating miRNAs showing altered expression between normal colonic (N), tubular adenoma (ADT), tubulovillous adenoma (ADTV) and colorectal cancer (CRC) matched plasma and tissue samples. Sixteen peripheral plasma and matched tissue biopsy samples (N n = 4; ADT n = 4; ADTV n = 4; CRC n = 4) were selected, and total RNA including miRNA fraction was isolated. MiRNAs from plasma samples were extracted using QIAamp Circulating Nucleic Acid Kit (Qiagen). Matched tissue-plasma miRNA microarray experiments were conducted by GeneChip® miRNA 3.0 Array (Affymetrix). RT-qPCR (microRNA Ready-to-use PCR Human Panel I + II; Exiqon) was used for validation. Characteristic miRNA expression alterations were observed in comparison of AD and CRC groups (miR-149*, miR-3196, miR-4687) in plasma samples. In the N vs. CRC comparison, significant overexpression of miR-612, miR-1296, miR-933, miR-937 and miR-1207 was detected by RT-PCR (p < 0.05). Similar expression pattern of these miRNAs were observed using microarray in tissue pairs, as well. Although miRNAs were also found in circulatory system in a lower concentration compared to tissues, expression patterns slightly overlapped between tissue and plasma samples. Detected circulating miRNA alterations may originate not only from the primer tumor but from other cell types including immune cells.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalPathology and Oncology Research
DOIs
Publication statusAccepted/In press - Oct 4 2017

Fingerprint

MicroRNAs
Colorectal Neoplasms
Adenoma
Polymerase Chain Reaction
Cardiovascular System
Nucleic Acids
RNA
Biopsy

Keywords

  • Circulating microRNA
  • Colorectal adenoma
  • Colorectal cancer
  • Microarray
  • microRNA
  • Plasma
  • Real-time PCR
  • Tissue

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oncology
  • Cancer Research

Cite this

Comparison of Circulating miRNAs Expression Alterations in Matched Tissue and Plasma Samples During Colorectal Cancer Progression. / Nagy, Zsófia Brigitta; Barták, Barbara Kinga; Kalmár, Alexandra; Galamb, O.; Wichmann, Barnabás; Dank, Magdolna; Igaz, P.; Tulassay, Z.; Molnár, B.

In: Pathology and Oncology Research, 04.10.2017, p. 1-9.

Research output: Contribution to journalArticle

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