Comparison of Botrytis cinerea populations isolated from two open-field cultivated host plants

Mojtaba Asadollahi, Erzsébet Fekete, Levente Karaffa, Michel Flipphi, Mariann árnyasi, Mahdi Esmaeili, Kálmán Zoltán Váczy, Erzsébet Sándor

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The necrotrophic fungus Botrytis cinerea is reported to infect more than 220 host plants worldwide. In phylogenetical-taxonomical terms, the pathogen is considered a complex of two cryptic species, group I and group II. We sampled populations of B. cinerea on sympatric strawberry and raspberry cultivars in the North-East of Hungary for three years during flowering and the harvest period. Four hundred and ninety group II B. cinerea isolates were analyzed for the current study. Three different data sets were generated: (i) PCR-RFLP patterns of the ADP-ATP translocase and nitrate reductase genes, (ii) MSB1 minisatellite sequence data, and (iii) the fragment sizes of five microsatellite loci. The structures of the different populations were similar as indicated by Nei's gene diversity and haplotype diversity. The F statistics (Fst, Gst), and the gene flow indicated ongoing differentiation within sympatric populations. The population genetic parameters were influenced by polymorphisms within the three data sets as assessed using Bayesian algorithms. Data Mining analysis pointed towards the five microsatellite loci as the most defining markers to study differentiation in the 490 isolates. The results suggest the occurrence of host-specific, sympatric divergence of generalist phytoparasites in perennial hosts.

Original languageEnglish
Pages (from-to)379-388
Number of pages10
JournalMicrobiological Research
Volume168
Issue number6
DOIs
Publication statusPublished - Jul 19 2013

Keywords

  • Botrytis cinerea
  • Data Mining
  • Genetic differentiation
  • Pathogen
  • Sympatric speciation

ASJC Scopus subject areas

  • Microbiology

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