Comparison of bioassays by testing whole soil and their water extract from contaminated sites

Laura Leitgib, Judit Kálmán, K. Gruiz

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The harmful effects of contaminants on the ecosystems and humans are characterised by their environmental toxicity. The aim of this study was to assess applicability and reliability of several environmental toxicity tests, comparing the result of the whole soils and their water extracts. In the study real contaminated soils were applied from three different inherited contaminated sites of organic and inorganic pollutants. The measured endpoints were the bioluminescence inhibition of Vibrio fischeri (bacterium), the dehydrogenase activity inhibition of Azomonas agilis (bacterium), the reproduction inhibition of Tetrahymena pyriformis (protozoon), and Panagrellus redivivus (nematode), the mortality of Folsomia candida (springtail), the root and shoot elongation inhibition of Sinapis alba (plant: white mustard) and the nitrification activity inhibition of an uncontaminated garden soil used as "test organism". Besides the standardised or widely used methods some new, direct contact ecotoxicity tests have been developed and introduced, which are useful for characterisation of the risk of contaminated soils due to their interactive nature. Soil no. 1 derived from a site polluted with transformer oil (PCB-free); Soil no. 2 originated from a site contaminated with mazout; Soil no. 3 was contaminated with toxic metals (Zn, Cd, Cu, Pb, As). In most cases, the interactive ecotoxicity tests indicated more harmful effect of the contaminated soil than the tests using soil extracts. The direct contact environmental toxicity tests are able to meet the requirements of environmental toxicology: reliability, sensibility, reproducibility, rapidity and low cost.

Original languageEnglish
Pages (from-to)428-434
Number of pages7
JournalChemosphere
Volume66
Issue number3
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Soil testing
Bioassay
Biological Assay
bioassay
Soil
Soils
Water
toxicity test
soil
Sinapis
water
Toxicity
Toxicity Tests
bioluminescence
bacterium
soil test
toxicology
organic pollutant
Pseudomonadaceae
Bacteria

Keywords

  • Environmental toxicity
  • Heavy metals
  • Interactive test methods
  • Mazout
  • Risk assessment
  • Transformer oil

ASJC Scopus subject areas

  • Environmental Chemistry
  • Environmental Science(all)

Cite this

Comparison of bioassays by testing whole soil and their water extract from contaminated sites. / Leitgib, Laura; Kálmán, Judit; Gruiz, K.

In: Chemosphere, Vol. 66, No. 3, 01.2007, p. 428-434.

Research output: Contribution to journalArticle

Leitgib, Laura ; Kálmán, Judit ; Gruiz, K. / Comparison of bioassays by testing whole soil and their water extract from contaminated sites. In: Chemosphere. 2007 ; Vol. 66, No. 3. pp. 428-434.
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