Comparison of biliary excretion of organic anions in mice and rats

Z. Gregus, Curtis D. Klaassen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Biliary excretion of cholephilic organic acids in anesthetized, male Swiss-Webster mice was compared to that in male Sprague-Dawley rats. The mouse excreted six of the eight compounds examined at a faster or equal rate than the rat. Indocyanine green, rose bengal, phenol-3,6-dibromsulphthalein disulfonate, and eosine were excreted in mice at a rate 120 to 460% higher than in rats. The excretion rates of bromcresol green and sulfobromophthalein glutathione conjugate were similar in the two species, whereas amaranth was excreted at a slightly lower rate in mice than in rats. Biliary excretion of sulfobromophthalein (BSP), especially its glutathione conjugate, was significantly lower in the mouse which corresponded to a difference in BSP-glutathione transferase activities between the two species (mouse, 0.97; rat, 1.35 μmol/min/g liver). The depression of bile production by cholestatic organic anions was stronger, and the stimulation of bile flow by choleretic acids was weaker in mice than in rats. Differences in biliary bile acid excretion (mouse, 3.62; rat, 1.42 μmol/kg/min), bile flow (mouse, 102; rat, 69 μl/kg/min), and liver weight (mouse, 57; rat, 38 g/kg) but not hepatic ligandin concentration (mouse, 132; rat, 214 nmol BSP/g liver) may explain the variations in the biliary organic anion excretion between mice and rats.

Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalToxicology and Applied Pharmacology
Volume63
Issue number1
DOIs
Publication statusPublished - Mar 30 1982

Fingerprint

Anions
Rats
Sulfobromophthalein
Liver
Bile
Glutathione Transferase
Hepatobiliary Elimination
Bromcresol Green
Cholagogues and Choleretics
Rose Bengal
Indocyanine Green
Organic acids
Acids
Eosine Yellowish-(YS)
Phenol
Bile Acids and Salts
Glutathione
Sprague Dawley Rats
Weights and Measures

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Comparison of biliary excretion of organic anions in mice and rats. / Gregus, Z.; Klaassen, Curtis D.

In: Toxicology and Applied Pharmacology, Vol. 63, No. 1, 30.03.1982, p. 13-20.

Research output: Contribution to journalArticle

@article{a9872f429bca4ab9b7d781efc51b5f9e,
title = "Comparison of biliary excretion of organic anions in mice and rats",
abstract = "Biliary excretion of cholephilic organic acids in anesthetized, male Swiss-Webster mice was compared to that in male Sprague-Dawley rats. The mouse excreted six of the eight compounds examined at a faster or equal rate than the rat. Indocyanine green, rose bengal, phenol-3,6-dibromsulphthalein disulfonate, and eosine were excreted in mice at a rate 120 to 460{\%} higher than in rats. The excretion rates of bromcresol green and sulfobromophthalein glutathione conjugate were similar in the two species, whereas amaranth was excreted at a slightly lower rate in mice than in rats. Biliary excretion of sulfobromophthalein (BSP), especially its glutathione conjugate, was significantly lower in the mouse which corresponded to a difference in BSP-glutathione transferase activities between the two species (mouse, 0.97; rat, 1.35 μmol/min/g liver). The depression of bile production by cholestatic organic anions was stronger, and the stimulation of bile flow by choleretic acids was weaker in mice than in rats. Differences in biliary bile acid excretion (mouse, 3.62; rat, 1.42 μmol/kg/min), bile flow (mouse, 102; rat, 69 μl/kg/min), and liver weight (mouse, 57; rat, 38 g/kg) but not hepatic ligandin concentration (mouse, 132; rat, 214 nmol BSP/g liver) may explain the variations in the biliary organic anion excretion between mice and rats.",
author = "Z. Gregus and Klaassen, {Curtis D.}",
year = "1982",
month = "3",
day = "30",
doi = "10.1016/0041-008X(82)90021-7",
language = "English",
volume = "63",
pages = "13--20",
journal = "Toxicology and Applied Pharmacology",
issn = "0041-008X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Comparison of biliary excretion of organic anions in mice and rats

AU - Gregus, Z.

AU - Klaassen, Curtis D.

PY - 1982/3/30

Y1 - 1982/3/30

N2 - Biliary excretion of cholephilic organic acids in anesthetized, male Swiss-Webster mice was compared to that in male Sprague-Dawley rats. The mouse excreted six of the eight compounds examined at a faster or equal rate than the rat. Indocyanine green, rose bengal, phenol-3,6-dibromsulphthalein disulfonate, and eosine were excreted in mice at a rate 120 to 460% higher than in rats. The excretion rates of bromcresol green and sulfobromophthalein glutathione conjugate were similar in the two species, whereas amaranth was excreted at a slightly lower rate in mice than in rats. Biliary excretion of sulfobromophthalein (BSP), especially its glutathione conjugate, was significantly lower in the mouse which corresponded to a difference in BSP-glutathione transferase activities between the two species (mouse, 0.97; rat, 1.35 μmol/min/g liver). The depression of bile production by cholestatic organic anions was stronger, and the stimulation of bile flow by choleretic acids was weaker in mice than in rats. Differences in biliary bile acid excretion (mouse, 3.62; rat, 1.42 μmol/kg/min), bile flow (mouse, 102; rat, 69 μl/kg/min), and liver weight (mouse, 57; rat, 38 g/kg) but not hepatic ligandin concentration (mouse, 132; rat, 214 nmol BSP/g liver) may explain the variations in the biliary organic anion excretion between mice and rats.

AB - Biliary excretion of cholephilic organic acids in anesthetized, male Swiss-Webster mice was compared to that in male Sprague-Dawley rats. The mouse excreted six of the eight compounds examined at a faster or equal rate than the rat. Indocyanine green, rose bengal, phenol-3,6-dibromsulphthalein disulfonate, and eosine were excreted in mice at a rate 120 to 460% higher than in rats. The excretion rates of bromcresol green and sulfobromophthalein glutathione conjugate were similar in the two species, whereas amaranth was excreted at a slightly lower rate in mice than in rats. Biliary excretion of sulfobromophthalein (BSP), especially its glutathione conjugate, was significantly lower in the mouse which corresponded to a difference in BSP-glutathione transferase activities between the two species (mouse, 0.97; rat, 1.35 μmol/min/g liver). The depression of bile production by cholestatic organic anions was stronger, and the stimulation of bile flow by choleretic acids was weaker in mice than in rats. Differences in biliary bile acid excretion (mouse, 3.62; rat, 1.42 μmol/kg/min), bile flow (mouse, 102; rat, 69 μl/kg/min), and liver weight (mouse, 57; rat, 38 g/kg) but not hepatic ligandin concentration (mouse, 132; rat, 214 nmol BSP/g liver) may explain the variations in the biliary organic anion excretion between mice and rats.

UR - http://www.scopus.com/inward/record.url?scp=0020083637&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020083637&partnerID=8YFLogxK

U2 - 10.1016/0041-008X(82)90021-7

DO - 10.1016/0041-008X(82)90021-7

M3 - Article

VL - 63

SP - 13

EP - 20

JO - Toxicology and Applied Pharmacology

JF - Toxicology and Applied Pharmacology

SN - 0041-008X

IS - 1

ER -