Comparison of a tissue tranglutaminase ELISA with the endomysium antibody test in the diagnosis of gluten-sensitive enteropathy

Miklós Sárdy, S. Kárpáti, F. Péterfy, K. Rásky, E. Tomsits, T. Zágoni, A. Horváth

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Tissue transglutaminase (tTG) has recently been found to be the major if not the only autoantigen of gluten-sensitive enteropathy (GSE). Objectives: To further determine the significance of this finding for diagnostic (screening) and follow-up purposes, we performed tTG-based ELISAs, and compared the results to the endomysium antibody test (EMA). Patients: We examined 120 serum samples from patients with celiac disease (CD) including 72 on a gluten-free diet (GFD) and eleven on a gluten challenge, 47 with dermatitis herpetiformis (DH) including 16 on a GFD and one on a gluten challenge, 96 with non-CD gastrointestinal diseases, and 117 with others; i. e. 380 serum samples altogether. Follow-up sera from 13 patients were included. Methods: Results of an ELISA with guinea pig liver tTG were compared with the EMA test using monkey esophagus. Inhibition of endomysial staining was performed with sera positive on the EMA test but negative with the guinea pig tTG ELISA. Results: The specificity and sensitivity of the tTG ELISA are high (98.6% and 92.5%). The serum IgA antibody titers against tTG decrease after introduction of a GFD. In one case, endomysial staining could not be inhibited. Conclusions: Our results show that the guinea pig tTG ELISA is suitable for use as a simple diagnostic, screening and follow-up method for GSE. Further studies are necessary to identify possible additional minor antigens in GSE.

Original languageEnglish
Pages (from-to)357-364
Number of pages8
JournalZeitschrift fur Gastroenterologie
Volume38
Issue number5
DOIs
Publication statusPublished - May 2000

Fingerprint

Celiac Disease
Enzyme-Linked Immunosorbent Assay
Antibodies
Gluten-Free Diet
Serum
Guinea Pigs
Glutens
Staining and Labeling
Dermatitis Herpetiformis
Gastrointestinal Diseases
Autoantigens
transglutaminase 2
Immunoglobulin A
Esophagus
Haplorhini
Antigens
Sensitivity and Specificity
Liver

Keywords

  • Celiac disease
  • Dermatitis herpetiformis
  • Endomysium antibodies
  • Enzyme-linked immunosorbent assay
  • Gluten-sensitive enteropathy
  • Indirect immunofluorescence
  • Tissue transglutaminase

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Comparison of a tissue tranglutaminase ELISA with the endomysium antibody test in the diagnosis of gluten-sensitive enteropathy. / Sárdy, Miklós; Kárpáti, S.; Péterfy, F.; Rásky, K.; Tomsits, E.; Zágoni, T.; Horváth, A.

In: Zeitschrift fur Gastroenterologie, Vol. 38, No. 5, 05.2000, p. 357-364.

Research output: Contribution to journalArticle

@article{842624470186425db4ee67e20dc995ca,
title = "Comparison of a tissue tranglutaminase ELISA with the endomysium antibody test in the diagnosis of gluten-sensitive enteropathy",
abstract = "Background: Tissue transglutaminase (tTG) has recently been found to be the major if not the only autoantigen of gluten-sensitive enteropathy (GSE). Objectives: To further determine the significance of this finding for diagnostic (screening) and follow-up purposes, we performed tTG-based ELISAs, and compared the results to the endomysium antibody test (EMA). Patients: We examined 120 serum samples from patients with celiac disease (CD) including 72 on a gluten-free diet (GFD) and eleven on a gluten challenge, 47 with dermatitis herpetiformis (DH) including 16 on a GFD and one on a gluten challenge, 96 with non-CD gastrointestinal diseases, and 117 with others; i. e. 380 serum samples altogether. Follow-up sera from 13 patients were included. Methods: Results of an ELISA with guinea pig liver tTG were compared with the EMA test using monkey esophagus. Inhibition of endomysial staining was performed with sera positive on the EMA test but negative with the guinea pig tTG ELISA. Results: The specificity and sensitivity of the tTG ELISA are high (98.6{\%} and 92.5{\%}). The serum IgA antibody titers against tTG decrease after introduction of a GFD. In one case, endomysial staining could not be inhibited. Conclusions: Our results show that the guinea pig tTG ELISA is suitable for use as a simple diagnostic, screening and follow-up method for GSE. Further studies are necessary to identify possible additional minor antigens in GSE.",
keywords = "Celiac disease, Dermatitis herpetiformis, Endomysium antibodies, Enzyme-linked immunosorbent assay, Gluten-sensitive enteropathy, Indirect immunofluorescence, Tissue transglutaminase",
author = "Mikl{\'o}s S{\'a}rdy and S. K{\'a}rp{\'a}ti and F. P{\'e}terfy and K. R{\'a}sky and E. Tomsits and T. Z{\'a}goni and A. Horv{\'a}th",
year = "2000",
month = "5",
doi = "10.1055/s-2000-14883",
language = "English",
volume = "38",
pages = "357--364",
journal = "Zeitschrift fur Gastroenterologie",
issn = "0044-2771",
publisher = "Karl Demeter Verlag GmbH",
number = "5",

}

TY - JOUR

T1 - Comparison of a tissue tranglutaminase ELISA with the endomysium antibody test in the diagnosis of gluten-sensitive enteropathy

AU - Sárdy, Miklós

AU - Kárpáti, S.

AU - Péterfy, F.

AU - Rásky, K.

AU - Tomsits, E.

AU - Zágoni, T.

AU - Horváth, A.

PY - 2000/5

Y1 - 2000/5

N2 - Background: Tissue transglutaminase (tTG) has recently been found to be the major if not the only autoantigen of gluten-sensitive enteropathy (GSE). Objectives: To further determine the significance of this finding for diagnostic (screening) and follow-up purposes, we performed tTG-based ELISAs, and compared the results to the endomysium antibody test (EMA). Patients: We examined 120 serum samples from patients with celiac disease (CD) including 72 on a gluten-free diet (GFD) and eleven on a gluten challenge, 47 with dermatitis herpetiformis (DH) including 16 on a GFD and one on a gluten challenge, 96 with non-CD gastrointestinal diseases, and 117 with others; i. e. 380 serum samples altogether. Follow-up sera from 13 patients were included. Methods: Results of an ELISA with guinea pig liver tTG were compared with the EMA test using monkey esophagus. Inhibition of endomysial staining was performed with sera positive on the EMA test but negative with the guinea pig tTG ELISA. Results: The specificity and sensitivity of the tTG ELISA are high (98.6% and 92.5%). The serum IgA antibody titers against tTG decrease after introduction of a GFD. In one case, endomysial staining could not be inhibited. Conclusions: Our results show that the guinea pig tTG ELISA is suitable for use as a simple diagnostic, screening and follow-up method for GSE. Further studies are necessary to identify possible additional minor antigens in GSE.

AB - Background: Tissue transglutaminase (tTG) has recently been found to be the major if not the only autoantigen of gluten-sensitive enteropathy (GSE). Objectives: To further determine the significance of this finding for diagnostic (screening) and follow-up purposes, we performed tTG-based ELISAs, and compared the results to the endomysium antibody test (EMA). Patients: We examined 120 serum samples from patients with celiac disease (CD) including 72 on a gluten-free diet (GFD) and eleven on a gluten challenge, 47 with dermatitis herpetiformis (DH) including 16 on a GFD and one on a gluten challenge, 96 with non-CD gastrointestinal diseases, and 117 with others; i. e. 380 serum samples altogether. Follow-up sera from 13 patients were included. Methods: Results of an ELISA with guinea pig liver tTG were compared with the EMA test using monkey esophagus. Inhibition of endomysial staining was performed with sera positive on the EMA test but negative with the guinea pig tTG ELISA. Results: The specificity and sensitivity of the tTG ELISA are high (98.6% and 92.5%). The serum IgA antibody titers against tTG decrease after introduction of a GFD. In one case, endomysial staining could not be inhibited. Conclusions: Our results show that the guinea pig tTG ELISA is suitable for use as a simple diagnostic, screening and follow-up method for GSE. Further studies are necessary to identify possible additional minor antigens in GSE.

KW - Celiac disease

KW - Dermatitis herpetiformis

KW - Endomysium antibodies

KW - Enzyme-linked immunosorbent assay

KW - Gluten-sensitive enteropathy

KW - Indirect immunofluorescence

KW - Tissue transglutaminase

UR - http://www.scopus.com/inward/record.url?scp=0033624059&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033624059&partnerID=8YFLogxK

U2 - 10.1055/s-2000-14883

DO - 10.1055/s-2000-14883

M3 - Article

C2 - 10875144

AN - SCOPUS:0033624059

VL - 38

SP - 357

EP - 364

JO - Zeitschrift fur Gastroenterologie

JF - Zeitschrift fur Gastroenterologie

SN - 0044-2771

IS - 5

ER -