Comparison between an intradermal skin test and allergen-specific IgE-ELISA for canine atopic dermatitis

N. Tarpataki, B. Bigler, P. Vajdovich, K. Vörös

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4 Citations (Scopus)

Abstract

The aim of this study was to compare the results of an intradermal skin test (IDST) with those of an allergen-specific IgE-ELISA in 210 dogs with atopic dermatitis. All the dogs had a clinical diagnosis of atopic dermatitis and underwent an IDST. The sera of all dogs were analysed for allergen-specific IgE by ELISA using the monoclonal antibody D9 against dog IgE. IDST was used as the standard assay. In both methods, the following antigens provided a positive test result: Dermatophagoides farinae, Acarus siro, Tyrophagus putrescentiae, ragweed, mugwort and Lepidoglyphus destructor. ELISA had an overall sensitivity of 82.4% and an overall specificity of 93.8%. The overall accuracy of the ELISA was 91.3%. The evaluated monoclonal D9 ELISA was found to be a reliable tool for the diagnosis of those allergens that cause clinical atopy, and can be recommended for use in dogs when immunotherapy is a therapeutic option.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalSchweizer Archiv fur Tierheilkunde
Volume150
Issue number3
DOIs
Publication statusPublished - Mar 1 2008

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Keywords

  • Atopic dermatitis
  • Canine IgE
  • ELISA
  • Intradermal skin test

ASJC Scopus subject areas

  • veterinary(all)

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