Comparing the optimum performance of the different modes of preparative liquid chromatography

Attila Felinger, Georges Guiochon

Research output: Contribution to journalArticle

90 Citations (Scopus)


A comparative study of the optimization of the different modes of the preparative separation of binary mixtures by liquid chromatography is presented. Band profiles were calculated by means of the equilibrium-dispersive model of chromatography in the cases of isocratic elution, gradient elution, and displacement chromatography. The objective function to be maximized was the product of the production rate and the recovery yield. The production rate was calculated using the same definition of the cycle time in all cases. This common definition accounts for column regeneration after each run in each mode of the separation. The calculations reveal that the number of experimental parameters to be adjusted to achieve optimum separations is relatively small. The major parameters are the loading factor and the number of theoretical plates, besides the displacer concentration in displacement chromatography, or the gradient steepness in gradient elution. The relative advantages of the different modes of preparative chromatography are discussed.

Original languageEnglish
Pages (from-to)59-74
Number of pages16
JournalJournal of Chromatography A
Issue number1
Publication statusPublished - Feb 13 1998


  • Band profiles
  • Optimisation
  • Preparative chromatography
  • Production rate

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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