Comparative viability studies on isolated gastric mucosal mixed cells and hepatoma and myeloma cell lines with ethanol, indomethacin and their combination

I. Szabo, B. Bodis, P. Németh, G. Mózsik

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The toxic effects of ethanol (EtOH), indomethacin (IND) and their combination were studied in vitro. The experiments were performed on freshly isolated gastric mucosal mixed cells and two types of stable cultured cells: Sp2/0-Ag14, which is a non-secreting mouse myeloma cell line, and Hep G2, which is a human hepatocellular carcinoma cell line. EtOH decreased the viability of all types of cells in a concentration-dependent manner. At all concentrations, the EtOH caused a greater decrease in the viability of gastric mucosal cells than in the viability of Sp2/0-Ag14 cells. IND had no effect on the viability of the cultured cells, when this was employed without any other aggressive factor, such as EtOH. When used in combination, IND aggravated the EtOH-induced cell injury. These results show that the endogenous prostaglandins may play a role in the maintenance of cell integrity in all three types of cells.

Original languageEnglish
Pages (from-to)21-28
Number of pages8
JournalInflammopharmacology
Volume5
Issue number1
Publication statusPublished - 1997

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Indomethacin
Hepatocellular Carcinoma
Stomach
Ethanol
Cell Line
Cultured Cells
Cell Survival
Poisons
Hep G2 Cells
Prostaglandins
Maintenance
Wounds and Injuries

Keywords

  • ethanol
  • Hep G2 cell line
  • indomethacin
  • isolated rat gastric mucosal cells
  • Sp2/0-Ag14 cell line

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

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AU - Szabo, I.

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AU - Németh, P.

AU - Mózsik, G.

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N2 - The toxic effects of ethanol (EtOH), indomethacin (IND) and their combination were studied in vitro. The experiments were performed on freshly isolated gastric mucosal mixed cells and two types of stable cultured cells: Sp2/0-Ag14, which is a non-secreting mouse myeloma cell line, and Hep G2, which is a human hepatocellular carcinoma cell line. EtOH decreased the viability of all types of cells in a concentration-dependent manner. At all concentrations, the EtOH caused a greater decrease in the viability of gastric mucosal cells than in the viability of Sp2/0-Ag14 cells. IND had no effect on the viability of the cultured cells, when this was employed without any other aggressive factor, such as EtOH. When used in combination, IND aggravated the EtOH-induced cell injury. These results show that the endogenous prostaglandins may play a role in the maintenance of cell integrity in all three types of cells.

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