Comparative susceptibility of larval instars and pupae of the western corn rootworm to infection by three entomopathogenic nematodes

B. Kurtz, I. Hiltpold, T. C J Turlings, U. Kuhlmann, S. Toepfer

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

As a first step towards the development of an ecologically rational control strategy against western corn rootworm (WCR; Diabrotica virgifera virgifera LeConte, Coleoptera: Chrysomelidae) in Europe, we compared the susceptibility of the soil living larvae and pupae of this maize pest to infection by three entomopathogenic nematode (EPN) species. In laboratory assays using sand-filled trays, Heterorhabditis bacteriophora Poinar and H. megidis Poinar, Jackson & Klein (both Rhabditida: Heterorhabditidae) caused comparable mortality among all three larval instars and pupae of D. v. virgifera. In soil-filled trays, H. bacteriophora was slightly more effective against third larval instars and pupae, and H. megidis against third larval instars, compared to other developmental stages. In both sand and soil, Steinernema feltiae (Filipjev) (Rh.: Steinernematidae) was least effective against second instars. In conclusion, all larval instars of D. v. virgifera show susceptibility to infection by all three nematodes tested. It is predicted that early application against young larval instars would be most effective at preventing root feeding damage by D. v. virgifera. Applications of nematodes just before or during the time period when third instars are predominant in the field are likely to increase control efficacy. According to our laboratory assays, H. bacteriophora and H. megidis appear to be the most promising candidates for testing in the field.

Original languageEnglish
Pages (from-to)255-262
Number of pages8
JournalBioControl
Volume54
Issue number2
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Diabrotica virgifera virgifera
entomopathogenic nematodes
pupae
instars
Heterorhabditis bacteriophora
infection
trays
Heterorhabditis megidis
Nematoda
sand
Heterorhabditidae
Steinernema feltiae
Steinernematidae
soil
assays
Chrysomelidae
developmental stages
pests
Coleoptera
corn

Keywords

  • Chrysomelidae
  • Coleoptera
  • Diabrotica virgifera virgifera
  • Heterorhabditis bacteriophora
  • Heterorhabditis megidis
  • Steinernema feltiae

ASJC Scopus subject areas

  • Insect Science
  • Agronomy and Crop Science

Cite this

Comparative susceptibility of larval instars and pupae of the western corn rootworm to infection by three entomopathogenic nematodes. / Kurtz, B.; Hiltpold, I.; Turlings, T. C J; Kuhlmann, U.; Toepfer, S.

In: BioControl, Vol. 54, No. 2, 04.2009, p. 255-262.

Research output: Contribution to journalArticle

Kurtz, B. ; Hiltpold, I. ; Turlings, T. C J ; Kuhlmann, U. ; Toepfer, S. / Comparative susceptibility of larval instars and pupae of the western corn rootworm to infection by three entomopathogenic nematodes. In: BioControl. 2009 ; Vol. 54, No. 2. pp. 255-262.
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