Comparative study on Pulsed Laser Deposition and Matrix Assisted Pulsed Laser Evaporation of urease thin films

Tomi Smausz, Gábor Megyeri, Renáta Kékesi, Csaba Vass, Eniko György, Felix Sima, Ion N. Mihailescu, Béla Hopp

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26 Citations (Scopus)

Abstract

Urease thin films were produced by Matrix Assisted Pulsed Laser Evaporation (MAPLE) and Pulsed Laser Deposition from two types of targets: frozen water solutions of urease with different concentrations (1-10% m/v) and pure urease pellets. The fluence of the ablating KrF excimer laser was varied between 300 and 2200 mJ/cm2. Fourier transform infrared spectra of the deposited films showed no difference as compared to the original urease. Morphologic studies proved that the films consist of a smooth "base" layer with embedded micrometer-sized droplets. Absorption-coefficient measurements contradicted the traditional "absorptive matrix" model for MAPLE deposition. The laser energy was absorbed by urease clusters leading to a local heating-up and evaporation of the frozen matrix from the uppermost layer accompanied by the release of dissolved urease molecules. Significant enzymatic activity of urease was preserved only during matrix assisted transfer.

Original languageEnglish
Pages (from-to)4299-4302
Number of pages4
JournalThin Solid Films
Volume517
Issue number15
DOIs
Publication statusPublished - Jun 1 2009

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Keywords

  • Enzyme
  • Laser deposition
  • Urease

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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