Comparative study on hydrolysis of triolein in reversed micellar system catalyzed by different types of lipases

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Abstract

Hydrolysis of triolein in AOT/isooctane reversed micelles by an sn-1,3-regioselective and a non-selective lipase were studied. Kinetics of the multistep reaction: decomposition of tri-, di- and monoacylglycerols and production of fatty acid were investigated separately. All the reactions was found to obey the Michaelis-Menten model and the apparent parameters (Michaelis-constants (Km) and maximal reaction rates (Vmax)) were determined both for non-selective and regioselective preparations.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalBiotechnology Techniques
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 1 1995

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ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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