Comparative study on determination of fumagillin in fish by normal and reversed phase chromatography

J. Fekete, Zs Romvári, I. Gebefügi, A. Kettrup

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Fumagillin is an antibiotic agent mostly used in the veterinary profession. A normal-phase liquid chromatographic method was developed for the determination of this drug in fish matrices. A hexane-dichloromethanedioxan-2-PrOH-acetic acid (43:43:9:5:0.1 v/v %) eluent was used on a Perkin Elmer silica gel column. For validation of the process a reversed-phase HPLC method was also developed (eluent: acetonitrile-water-bicyclohexylamine 70:30:0.05 v/v %, with Spherisorb ODS, as stationary phase). Recovery is about 100 % and the limit of detection is 5 ng g-1 for meat samples.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalChromatographia
Volume48
Issue number1-2
DOIs
Publication statusPublished - Jul 29 1998

Keywords

  • Column liquid chromatography
  • Fumagillin in fish samples
  • Normal- and reversed-phase LC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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