Comparative study of the chiral resolution of β-blockers on cellulose tris (3,5-dimethylphenylcarbamate) phases in normal and reversed phase modes

Martin G. Schmid, Orsolya Gecse, Zoltan Szabo, Ferenc Kilár, Gerald Gübitz, Imran Ali, Hassan Y. Aboul-Enein

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The chiral separation of β-blockers on cellulose tris (3,5-dimethylphenylcarbamate) columns is described. The separation power of a Chiracel OD normal phase column, a Chiracel OD-RH reversed phase column, and a Chiracel ODRH reversed phase narrow-bore column were compared. Hexane-2-propanol-diethylamine mixtures were used as mobile phase in the normal phase mode and sodium perchlorate buffer containing different amounts of acetonitrile in the reversed phase mode. Sixteen μ-blockers were resolved under normal phase conditions and eleven under reversed phase conditions. The values of α and Rs on normal phase column ranged from 4.46 to 1.10 and 10.04 to 0.97, respectively. The values of α and Rs on reversed phase columns varied from 4.04 to 1.04 and 3.98 to 0.40 (on normal bore column) and 4.36 to 1.06 and 2.26 to 0.40 (on narrow bore column).

Original languageEnglish
Pages (from-to)2493-2504
Number of pages12
JournalJournal of Liquid Chromatography and Related Technologies
Volume24
Issue number16
DOIs
Publication statusPublished - Oct 16 2001

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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