The chiral separation of β-blockers on cellulose tris (3,5-dimethylphenylcarbamate) columns is described. The separation power of a Chiracel OD normal phase column, a Chiracel OD-RH reversed phase column, and a Chiracel ODRH reversed phase narrow-bore column were compared. Hexane-2-propanol-diethylamine mixtures were used as mobile phase in the normal phase mode and sodium perchlorate buffer containing different amounts of acetonitrile in the reversed phase mode. Sixteen μ-blockers were resolved under normal phase conditions and eleven under reversed phase conditions. The values of α and Rs on normal phase column ranged from 4.46 to 1.10 and 10.04 to 0.97, respectively. The values of α and Rs on reversed phase columns varied from 4.04 to 1.04 and 3.98 to 0.40 (on normal bore column) and 4.36 to 1.06 and 2.26 to 0.40 (on narrow bore column).
|Number of pages||12|
|Journal||Journal of Liquid Chromatography and Related Technologies|
|Publication status||Published - Oct 16 2001|
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
- Clinical Biochemistry