Comparative study of cyanotoxins affecting cytoskeletal and chromatin structures in CHO-K1 cells

Mariann Gácsi, Otilia Antal, G. Vasas, Csaba Máthé, G. Borbély, Martin L. Saker, János Gyori, A. Farkas, A. Vehovszky, G. Bánfalvi

Research output: Contribution to journalArticle

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Abstract

In this study we compared the effects of the two frequently occuring and most dangerous cyanobacterial toxins on the cellular organization of microfilaments, microtubules and on the chromatin structure in Chinese hamster ovary (CHO-K1) cells. These compounds are the widely known microcystin-LR (MC-LR) and cylindrospermopsin (CYN) classified as the highest-priority cyanotoxin. Toxic effects were tested in a concentration and time dependent manner. The hepatotoxic MC-LR did not cause significant cytotoxicity on CHO-K1 cells under 20 μM, but caused apoptotic changes at higher concentrations. Apoptotic shrinkage was associated with the shortening and loss of actin filaments and with a concentration dependent depolymerization of microtubules. No necrosis was observed over the concentration range (1-50 μM MC-LR) tested. Cylindrospermopsin did cause apoptosis at low concentrations (1-2 μM) and over short exposure periods (12 h). Necrosis was observed at higher concentrations (5-10 μM) and following longer exposure periods (24 or 48 h). Cyanotoxins also affected the chromatin structure. The condensation process was inhibited by MC-LR at a later stage and manifested as broken elongated prechromosomes. CYN inhibited chromatin condensation at the early fibrillary stage leading to blurred fluorescent images of apoptotic bodies and preventing the formation of metaphase chromosomes. Cylindrospermopsin exhibited a more pronounced toxic effect causing cytoskeletal and nuclear changes as well as apoptotic and necrotic alterations.

Original languageEnglish
Pages (from-to)710-718
Number of pages9
JournalToxicology in Vitro
Volume23
Issue number4
DOIs
Publication statusPublished - Jun 2009

Fingerprint

CHO Cells
Chromatin
Poisons
Actin Cytoskeleton
Microtubules
Condensation
Necrosis
Depolymerization
Metaphase
Cytotoxicity
Chromosomes
Cricetulus
Actins
Ovary
Apoptosis
cylindrospermopsin
cyanoginosin LR

Keywords

  • Actin
  • Apoptosis
  • Cylindrospermopsin
  • Fluorescence microscopy
  • Microcystin
  • Necrosis
  • Tubulin

ASJC Scopus subject areas

  • Toxicology

Cite this

Comparative study of cyanotoxins affecting cytoskeletal and chromatin structures in CHO-K1 cells. / Gácsi, Mariann; Antal, Otilia; Vasas, G.; Máthé, Csaba; Borbély, G.; Saker, Martin L.; Gyori, János; Farkas, A.; Vehovszky, A.; Bánfalvi, G.

In: Toxicology in Vitro, Vol. 23, No. 4, 06.2009, p. 710-718.

Research output: Contribution to journalArticle

Gácsi, Mariann ; Antal, Otilia ; Vasas, G. ; Máthé, Csaba ; Borbély, G. ; Saker, Martin L. ; Gyori, János ; Farkas, A. ; Vehovszky, A. ; Bánfalvi, G. / Comparative study of cyanotoxins affecting cytoskeletal and chromatin structures in CHO-K1 cells. In: Toxicology in Vitro. 2009 ; Vol. 23, No. 4. pp. 710-718.
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