Comparative studies with six extenders for sperm cryopreservation in the cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta)

Yahui Li, Kejun Cai, Jian Li, A. Dinnyés, Weizhi Ji

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Ejaculated spermatozoa from cynomolgus monkeys and rhesus monkeys were frozen in straws with six different extenders (TTE, DM, mDM, LG-DM, G-DM, and TCG) containing glycerol. Sperm motility and head membrane and acrosomal integrity were evaluated after freezing and thawing, and the cryoprotective effects were compared among the extenders and the two species studied. The results showed that sperm motility and motility recovery with the six extenders were comparable for the cynomolgus and rhesus monkeys. There was no significant difference in sperm motility and head membrane integrity among the six extenders in either the cynomolgus or rhesus monkeys (P > 0.05). However, a slightly but statistically lower percentage of acrosomal integrity was found with TCG in both species compared to the other extenders (P <0.05). These findings demonstrate that TTE, DM, mDM, LG-DM, G-DM, and TCG are equally suitable extenders for the cryopreservation of spermatozoa from cynomolgus and rhesus monkeys.

Original languageEnglish
Pages (from-to)39-49
Number of pages11
JournalAmerican Journal of Primatology
Volume68
Issue number1
DOIs
Publication statusPublished - Jan 2006

Fingerprint

cryopreservation
Macaca fascicularis
motility
Macaca mulatta
sperm
comparative study
sperm motility
spermatozoa
membrane
thawing
straw
freezing
glycerol

Keywords

  • Cryopreservation
  • Extender
  • Macaca fascicularis
  • Macaca, mulatta

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Comparative studies with six extenders for sperm cryopreservation in the cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta). / Li, Yahui; Cai, Kejun; Li, Jian; Dinnyés, A.; Ji, Weizhi.

In: American Journal of Primatology, Vol. 68, No. 1, 01.2006, p. 39-49.

Research output: Contribution to journalArticle

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