Comparative studies on platelet membrane preparations

M. Hasitz, V. Harsanyi, Z. Racz, S. R. Hollan

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For the isolation of the platelet plasma and the cytoplasmatic membranes, the following methods were applied: glycerol-osmotic lysis, combined with sonication; glycerol-osmotic lysis carried out on fresh or frozen platelets; and detergent treatment followed by separation of the protein fractions insoluble at low ionic strength. The detergents used were Triton X-100, Nonidet P-40, Na-deoxycholate and digitonin. The protein fractions were compared by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE).

Original languageEnglish
Pages (from-to)343-352
Number of pages10
Issue number3-4
Publication statusPublished - Dec 1 1976

ASJC Scopus subject areas

  • Hematology

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    Hasitz, M., Harsanyi, V., Racz, Z., & Hollan, S. R. (1976). Comparative studies on platelet membrane preparations. Haematologia, 10(3-4), 343-352.