Comparative studies on platelet membrane preparations

M. Hasitz, V. Harsanyi, Z. Racz, S. Hollán

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

For the isolation of the platelet plasma and the cytoplasmatic membranes, the following methods were applied: glycerol-osmotic lysis, combined with sonication; glycerol-osmotic lysis carried out on fresh or frozen platelets; and detergent treatment followed by separation of the protein fractions insoluble at low ionic strength. The detergents used were Triton X-100, Nonidet P-40, Na-deoxycholate and digitonin. The protein fractions were compared by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE).

Original languageEnglish
Pages (from-to)343-352
Number of pages10
JournalHaematologia
Volume10
Issue number3-4
Publication statusPublished - 1976

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Detergents
Glycerol
Blood Platelets
Digitonin
Sonication
Deoxycholic Acid
Membranes
Octoxynol
Sodium Dodecyl Sulfate
Osmolar Concentration
Polyacrylamide Gel Electrophoresis
Proteins
Cell Membrane
Nonidet P-40

ASJC Scopus subject areas

  • Hematology

Cite this

Hasitz, M., Harsanyi, V., Racz, Z., & Hollán, S. (1976). Comparative studies on platelet membrane preparations. Haematologia, 10(3-4), 343-352.

Comparative studies on platelet membrane preparations. / Hasitz, M.; Harsanyi, V.; Racz, Z.; Hollán, S.

In: Haematologia, Vol. 10, No. 3-4, 1976, p. 343-352.

Research output: Contribution to journalArticle

Hasitz, M, Harsanyi, V, Racz, Z & Hollán, S 1976, 'Comparative studies on platelet membrane preparations', Haematologia, vol. 10, no. 3-4, pp. 343-352.
Hasitz M, Harsanyi V, Racz Z, Hollán S. Comparative studies on platelet membrane preparations. Haematologia. 1976;10(3-4):343-352.
Hasitz, M. ; Harsanyi, V. ; Racz, Z. ; Hollán, S. / Comparative studies on platelet membrane preparations. In: Haematologia. 1976 ; Vol. 10, No. 3-4. pp. 343-352.
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